1,2-Dioleoyl-sn-glycero-3-PG (sodium salt)
(Synonyms: 1,2-二油酰-SN-甘油基-3-磷酸-RAC-甘油钠盐) 目录号 : GC41816
1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) 是一种阴离子磷脂，在细胞膜的结构和功能中起着重要作用
Cas No.:67254-28-8
Sample solution is provided at 25 µL, 10mM.
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1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) is an anionic phospholipid that plays a significant role in the structure and function of cell membranes[1]. 1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) is wildly used in the preparation of liposomes and other artificial membrane systems for studying the behavior of membrane proteins[2], drug delivery mechanisms[3][4], and the structure and function of cell membranes[5].
1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) can suppress the production of inflammatory mediators induced by recombinant S100A9 through both TLR2 and TLR4 pathways. In RAW264.7 cells, treatment with 100mg/ml DOPG and 2.5mg/ml S100A9 for 2 hours significantly inhibit the expression of IL-1β, IL-6, and TNF-α (tumor necrosis factor-α)[6].
1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) in water (prepared by sonicating a 1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) lipid film in deionized water) applied to the imiquimod (IMQ) treated mouse skin can inhibited IMQ-induced psoriasiform phenotype such as ear thickness and epidermal hyperplasia[6].
References:
[1]. Dinh BT, Price SE, Majul A, et al. Diazeniumdiolate reactivity in model membrane systems. Nitric Oxide. 2008 Mar;18(2):113-21. doi: 10.1016/j.niox.2007.11.001. Epub 2007 Nov 21. PMID: 18068133; PMCID: PMC2268087.
[2]. Balali-Mood K, Harroun TA, Bradshaw JP. Molecular dynamics simulations of a mixed DOPC/DOPG bilayer. Eur Phys J E Soft Matter. 2003 Nov;12 Suppl 1:S135-40. doi: 10.1140/epjed/e2003-01-031-3. Epub 2003 Nov 5. PMID: 15011033.
[3]. Srinivasan C, Burgess DJ. Optimization and characterization of anionic lipoplexes for gene delivery. J Control Release. 2009 May 21;136(1):62-70. doi: 10.1016/j.jconrel.2009.01.022. Epub 2009 Feb 5. PMID: 19331848.
[4]. Zhou S, Luo Y, Lovell JF. Vaccine approaches for antigen capture by liposomes. Expert Rev Vaccines. 2023 Jan-Dec;22(1):1022-1040. doi: 10.1080/14760584.2023.2274479. Epub 2023 Nov 6. PMID: 37878481; PMCID: PMC10872528.
[5]. Dargel C, Gräbitz-Bräuer F, Geisler R, et al. Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM. Molecules. 2021 Aug 16;26(16):4959. doi: 10.3390/molecules26164959. PMID: 34443547; PMCID: PMC8399256.
[6]. Choudhary V, Uaratanawong R, Patel RR, et al. Phosphatidylglycerol Inhibits Toll-Like Receptor-Mediated Inflammation by Danger-Associated Molecular Patterns. J Invest Dermatol. 2019 Apr;139(4):868-877. doi: 10.1016/j.jid.2018.10.021. Epub 2018 Oct 31. PMID: 30391260; PMCID: PMC7309510.
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)是一种阴离子磷脂，在细胞膜的结构和功能中起着重要作用[1]。1,2-Dioleoyl-sn-glycero-3-PG (sodium salt) 广泛用于制备脂质体和其他人工膜系统，以研究膜蛋白的行为[2]、药物传递机制[3] [4]以及细胞膜的结构和功能[5]。
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)可以通过TLR2和TLR4途径抑制重组S100A9蛋白引起的炎性介质的产生。在RAW264.7细胞中用100mg/ml的1,2-Dioleoyl-sn-glycero-3-PG (sodium salt)和2.5mg/ml的S100A9处理2小时可以显著抑制IL-1β、IL-6和TNF-α (肿瘤坏死因子-α) 的表达[6]。
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)溶液（通过在去离子水中超声处理1,2-Dioleoyl-sn-glycero-3-PG(sodium salt)脂质膜制备）涂抹在咪喹莫特（IMQ）处理的小鼠皮肤上，可以抑制IMQ引起的银屑病样表型，如耳朵增厚和表皮增生[6]。
| Cell experiment [1]: | |
|
Cell lines |
RAW264.7 cells |
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Preparation Method |
RAW264.7 cells were treated with 100mg/ml 1,2-Dioleoyl-sn-glycero-3-PG(sodium salt) and 2.5mg/ml S100A9 for 2 hours. The expression level of the inflammatory mediators IL-1b, IL-6, and TNF-a were measured by quantitative real-time reverse transcriptase-PCR with GAPDH used as the housekeeping gene. |
|
Reaction Conditions |
100mg/ml, 2h |
|
Applications |
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt) can inhibited S100A9-induced inflammatory mediator expression in RAW264.7 cells. |
| Animal experiment [2]: | |
|
Animal models |
Psoriasis mouse |
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Preparation Method |
Mice were treated with vehicle (petroleum jelly) or IMQ daily for 5 days in the morning. In the afternoon, water or 1,2-Dioleoyl-sn-glycero-3-PG(sodium salt) solution was applied to the treated skin. Then the Psoriasis Area and Severity Index score was estimated in a blinded manner. |
|
Dosage form |
5 days, Topical application |
|
Applications |
1,2-Dioleoyl-sn-glycero-3-PG(sodium salt) applied topically in the form of liposomes can inhibited IMQ-induced psoriasiform phenotype. |
|
References: [1]. Choudhary V, Uaratanawong R, Patel RR, et al. Phosphatidylglycerol Inhibits Toll-Like Receptor-Mediated Inflammation by Danger-Associated Molecular Patterns. J Invest Dermatol. 2019 Apr;139(4):868-877. doi: 10.1016/j.jid.2018.10.021. Epub 2018 Oct 31. PMID: 30391260; PMCID: PMC7309510. |
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| Cas No. | 67254-28-8 | SDF | |
| 别名 | 1,2-二油酰-SN-甘油基-3-磷酸-RAC-甘油钠盐 | ||
| Canonical SMILES | CCCCCCCC/C=C\CCCCCCCC(O[C@@H](COP(OCC(O)CO)([O-])=O)COC(CCCCCCC/C=C\CCCCCCCC)=O)=O.[Na+] | ||
| 分子式 | C42H78O10P•Na | 分子量 | 797 |
| 溶解度 | Chloroform: 2 mg/ml | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2547 mL | 6.2735 mL | 12.5471 mL |
| 5 mM | 0.2509 mL | 1.2547 mL | 2.5094 mL |
| 10 mM | 0.1255 mL | 0.6274 mL | 1.2547 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet