1,2-Dipalmitoyl-sn-glycero-O-ethyl-3-PC (chloride)
(Synonyms: 1,2-二棕榈酰-SN-甘油-3-乙基磷酸胆碱,氯盐) 目录号 : GC41827
A cationic phosphocholine
Cas No.:328250-18-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
1,2-Dipalmitoyl-sn-glycero-O-ethyl-3-PC is a phospholipid containing the saturated long-chain (16:0) stearic acid inserted at the sn-1 and sn-2 positions and an alkyl group on the phosphate oxygen of the polar headgroup. Cationic phospholipids such as this have proved useful as DNA transfection agents and for studies of surface charge density within lipid bilayers.
| Cas No. | 328250-18-6 | SDF | |
| 别名 | 1,2-二棕榈酰-SN-甘油-3-乙基磷酸胆碱,氯盐 | ||
| Canonical SMILES | O=C(CCCCCCCCCCCCCCC)OC[C@@H](OC(CCCCCCCCCCCCCCC)=O)COP(OCC[N+](C)(C)C)(OCC)=O.[Cl-] | ||
| 分子式 | C42H85NO8P•Cl | 分子量 | 798.6 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2522 mL | 6.261 mL | 12.5219 mL |
| 5 mM | 0.2504 mL | 1.2522 mL | 2.5044 mL |
| 10 mM | 0.1252 mL | 0.6261 mL | 1.2522 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
chloride to the rescue
J Biol Chem 2019 Jul 26;294(30):11402-11403.PMID:31350284DOI:10.1074/jbc.H119.009687.
On the fiftieth anniversary of the discovery of the Ser-His-Asp catalytic triad, perhaps the most unusual variation on the textbook classic is described: An incomplete catalytic triad in a hydrolase is rescued by a chloride ion (Fig. 1). Structural and functional data provide compelling evidence that the active site of a phospholipase from Vibrio vulnificus employs the anion in place of the commonly observed Asp, reminding us that even well-trodden scientific ground has surprises in store.
Coacervation between Two Positively Charged Poly(ionic liquid)s
Macromol Rapid Commun 2022 Sep;43(18):e2200191.PMID:35632991DOI:10.1002/marc.202200191.
Complex coacervates are usually formed through electrostatic attraction between oppositely charged polyelectrolytes, with a few exceptions such as coacervates of like-charge proteins and polyelectrolytes, both in vivo and in vitro. Understanding of the preparation and mechanisms of these coacervates is limited. Here, a positively charged poly(ionic liquid), poly(1-vinyl-3-benzylimidazolium chloride) (PILben), is designed that bears benzene rings in repeating units. Fluidic coacervates are prepared by mixing the PILben aqueous solution with a like-charge poly(ionic liquid) named poly(dimethyl diallyl ammonium chloride) (PDDA). The effects of polymer concentration, temperature, and ionic strength in the PILben-PDDA coacervate are studied. Raman spectroscopy and 2D 1 H-13 C heteronuclear single quantum coherence (1 H-13 C HSQC) characterizations verify that the coacervate formation benefits from the cation-π interaction between PILben and PDDA. This work provides principles and understandings of designing coacervates derived from like-charge poly(ionic liquids) with high charge density.
Chlorine cycling and the fate of Cl in terrestrial environments
Environ Sci Pollut Res Int 2021 Feb;28(7):7691-7709.PMID:33400105DOI:10.1007/s11356-020-12144-6.
Chlorine (Cl) in the terrestrial environment is of interest from multiple perspectives, including the use of chloride as a tracer for water flow and contaminant transport, organochlorine pollutants, Cl cycling, radioactive waste (radioecology; 36Cl is of large concern) and plant science (Cl as essential element for living plants). During the past decades, there has been a rapid development towards improved understanding of the terrestrial Cl cycle. There is a ubiquitous and extensive natural chlorination of organic matter in terrestrial ecosystems where naturally formed chlorinated organic compounds (Clorg) in soil frequently exceed the abundance of chloride. chloride dominates import and export from terrestrial ecosystems while soil Clorg and biomass Cl can dominate the standing stock Cl. This has important implications for Cl transport, as chloride will enter the Cl pools resulting in prolonged residence times. Clearly, these pools must be considered separately in future monitoring programs addressing Cl cycling. Moreover, there are indications that (1) large amounts of Cl can accumulate in biomass, in some cases representing the main Cl pool; (2) emissions of volatile organic chlorines could be a significant export pathway of Cl and (3) that there is a production of Clorg in tissues of, e.g. plants and animals and that Cl can accumulate as, e.g. chlorinated fatty acids in organisms. Yet, data focusing on ecosystem perspectives and combined spatiotemporal variability regarding various Cl pools are still scarce, and the processes and ecological roles of the extensive biological Cl cycling are still poorly understood.
Structures and Spectra of Halide Hydrate Clusters in the Solid State: A Link between the Gas Phase and Solution State
Chempluschem 2022 Feb;87(2):e202100535.PMID:35195348DOI:10.1002/cplu.202100535.
This Review describes and assesses known solid-state examples of halide hydrates that are discrete in nature. Most of these are chloride hydrates, and most discrete clusters are dihalides, with very few mono- or multi-halide species found in the solid state. Polymeric chloride hydrates, on the other hand, are mostly 2D layered structures. We also observe that there is a gap in the chloride:water ratio between 8-20 waters per chloride. Isolated clusters can be found with 1-3 waters per chloride, 2D layers with 2-8 waters, and 3D semiclathrates with 20-38 waters. However, 1D chains comprise only 1-2 waters per chloride. [Cl(H2 O)]- is the only species found in both the solid state and gas phase and is also the only halide hydrate with a free OH group. Infrared spectra in the ν(OH) region are distinctive and useful for identification. Agreement between computed (gas phase) and experimentally-observed solid state structures and their vibrational spectra gives us confidence that discrete halide hydrate species observed in the solid state provide a useful link between gas phase species and structural motifs of halide hydrates in solution, especially microsolvated ion-pairs.
Development and biological applications of chloride-sensitive fluorescent indicators
Am J Physiol 1990 Sep;259(3 Pt 1):C375-88.PMID:2205105DOI:10.1152/ajpcell.1990.259.3.C375.
chloride movement across cell plasma and internal membranes, is of central importance for regulation of cell volume and pH, vectorial salt movement in epithelia, and, probably, intracellular traffic. Quinolinium-based chloride-sensitive fluorescent indicators provide a new approach to study chloride transport mechanisms and regulation that is complementary to 36Cl tracer methods, intracellular microelectrodes, and patch clamp. Indicator fluorescence is quenched by chloride by a collisional mechanism with Stern-Volmer constants of up to 220 M-1. Fluorescence is quenched selectively by chloride in physiological systems and responds to changes in chloride concentration in under 1 ms. The indicators are nontoxic and can be loaded into living cells for continuous measurement of intracellular chloride concentration by single-cell fluorescence microscopy. In this review, the structure-activity relationships for chloride-sensitive fluorescent indicators are described. Methodology for measurement of chloride transport in isolated vesicle and liposome systems and in intact cells is evaluated critically by use of examples from epithelial cell physiology. Future directions for synthesis of tailored chloride-sensitive indicators and new applications of indicators for studies of transport regulation and intracellular ion gradients are proposed.