1,2-Distearoyl-sn-glycero-3-PC
(Synonyms: 二硬脂酰磷脂酰胆碱,1,2-Distearoyl-sn-glycero-3-PC; DSPC) 目录号 : GC41832
1,2-Distearoyl-sn-glycero-3-PC(DSPC)是一种脂质，可用于合成脂质体、胶束和其它类型的人造膜。
Cas No.:816-94-4
Sample solution is provided at 25 µL, 10mM.
1,2-Distearoyl-sn-glycero-3-PC (DSPC) is a lipid that can be used to synthesize liposomes, micelles and other types of artificial membranes[1]. 1,2-Distaroyl-sn-glycero-3-PC is a metabolite produced in Saccharomyces cerevisiae[2]. Mixed lipid monolayers containing 1,2-Distearoyl-sn-glycero-3-PC can bind to calcium-mediated DNA[3].
1,2-Distearoyl-sn-glycero-3-PC and CdSe/ZnS QD were prepared into liposome formulations to encapsulate the drug TNT, and were treated with HeLa cells. Compared with free TNT, it was easier to be taken up and internalized by cells, significantly improving Cytotoxic effects[4]. 1,2-Distaroyl-sn-glycero-3-PC encapsulated liposomes treated HeLa and 104C1 cells to promote Chlamydia trachomatis Ct D infection[5].
References:
[1] Caldo K M P, Shen W, Xu Y, et al. Diacylglycerol acyltransferase 1 is activated by phosphatidate and inhibited by SnRK1‐catalyzed phosphorylation[J]. The Plant Journal, 2018, 96(2): 287-299.
[2] de Kroon A I P M. Metabolism of phosphatidylcholine and its implications for lipid acyl chain composition in Saccharomyces cerevisiae[J]. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 2007, 1771(3): 343-352.
[3] Dabkowska A P, Barlow D J, Clifton L A, et al. Calcium-mediated binding of DNA to 1, 2-distearoyl-sn-glycero-3-phosphocholine-containing mixed lipid monolayers[J]. Soft matter, 2014, 10(11): 1685-1695.
[4] Seleci M, Ag Seleci D, Scheper T, et al. Theranostic liposome–nanoparticle hybrids for drug delivery and bioimaging[J]. International journal of molecular sciences, 2017, 18(7): 1415.
[5] Wali S, Gupta R, Yu J J, et al. Guinea pig genital tract lipidome reveals in vivo and in vitro regulation of phosphatidylcholine 16: 0/18: 1 and contribution to Chlamydia trachomatis serovar D infectivity[J]. Metabolomics, 2016, 12: 1-11.
1,2-Distearoyl-sn-glycero-3-PC(DSPC)是一种脂质，可用于合成脂质体、胶束和其它类型的人造膜[1]。1,2-Distearoyl-sn-glycero-3-PC是在酿酒酵母中产生的代谢产物[2]。含1,2-Distearoyl-sn-glycero-3-PC的混合脂质单层可与钙介导的DNA结合[3]。
1,2-Distearoyl-sn-glycero-3-PC与CdSe/ZnS QD制备成脂质体制剂包封药物TNT，处理HeLa细胞，相较于游离TNT，更易被细胞摄取和内化，显著提高了细胞毒性作用[4]。1,2-Distearoyl-sn-glycero-3-PC封装脂质体，处理Hela和 104C1细胞，促进沙眼衣原体Ct D感染[5]。
| Cas No. | 816-94-4 | SDF | |
| 别名 | 二硬脂酰磷脂酰胆碱,1,2-Distearoyl-sn-glycero-3-PC; DSPC | ||
| Canonical SMILES | O=C(CCCCCCCCCCCCCCCCC)OC[C@@H](OC(CCCCCCCCCCCCCCCCC)=O)COP(OCC[N+](C)(C)C)([O-])=O | ||
| 分子式 | C44H88NO8P | 分子量 | 790.2 |
| 溶解度 | ≤25mg/ml in ethanol | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2655 mL | 6.3275 mL | 12.655 mL |
| 5 mM | 0.2531 mL | 1.2655 mL | 2.531 mL |
| 10 mM | 0.1266 mL | 0.6328 mL | 1.2655 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet