1,3-Dimethyluric acid
(Synonyms: 1,3-二甲基尿酸) 目录号 : GC30749
1,3-二甲基尿酸（1,3-Dimethyluric acid）是一种氧嘌呤，它是 7,9-二氢-1H-嘌呤-2,6,8(3H)-三酮，在 N-1 和 N-3 被甲基取代。是人体茶碱代谢的产物。1,3-二甲基尿酸是尿路结石中的嘌呤成分之一。
Cas No.:944-73-0
Sample solution is provided at 25 µL, 10mM.
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1,3-Dimethyluric acid is an active metabolite of the methylxanthine alkaloids caffeine and theophylline .1 It is formed from caffeine and theophylline by the cytochrome P450 (CYP) isoforms CYP1A2 and CYP2E1.2,3 1,3-Dimethyluric acid (500 ?M) scavenges hydroxyl radicals in a cell-free assay and inhibits t-butyl hydroperoxide-induced lipid peroxidation by 77% in isolated human erythrocyte membranes.4 It induces contractions in isolated rabbit duodenal, jejunal, and ileal preparations, but induces relaxation in isolated rabbit ascending colon preparations, in a concentration-dependent manner.5 Intracerebral administration of 1,3-dimethyluric acid induces clonic convulsions in mice (ED50 = 360 nmol/animal).6 It has been found in urinary caliculi.7
1.Cornish, H.H., and Christman, A.A.A study of the metabolism of theobromine, theophylline, and caffeine in manJ. Biol. Chem.228(1)315-323(1957) 2.Caubet, M.-S., Elbast, W., Dubuc, M.-C., et al.Analysis of urinary caffeine metabolites by HPLC-DAD: The use of metabolic ratios to assess CYP1A2 enzyme activityJ. Pharm. Biomed. Anal.27(1-2)261-270(2002) 3.Fuhr, U., Doehmer, J., Battula, N., et al.Biotransformation of methylxanthines in mammalian cell lines genetically engineered for expression of single cytochrome P450 isoforms. Allocation of metabolic pathways to isoforms and inhibitory effects of quinolonesToxicology82(1-3)169-189(1993) 4.Bhat, V.B., Sridhar, G.R., and Madyastha, K.M.Efficient scavenging of hydroxyl radicals and inhibition of lipid peroxidation by novel analogues of 1,3,7-trimethyluric acidLife Sci.70(4)381-393(2001) 5.Psarra, T.A., Batzias, G.C., Peeters, T.L., et al.Theophylline and its metabolites produce a stimulating cholinergic effect on the small intestine and a nonadrenergic noncholinergic relaxing effect on the colon: A comparative study in the rabbit intestineJ. Vet. Pharmacol. Ther.30(6)541-519(2007) 6.Yamamoto, K., Toyama, E., Kawakami, J., et al.Neurotoxic convulsions induced by theophylline and its metabolites in miceBiol. Pharm. Bull.19(6)869-872(1996) 7.Safranow, K., and Machoy, Z.Methylated purines in urinary stonesClin. Chem.51(8)1493-1498(2005)
| Cas No. | 944-73-0 | SDF | |
| 别名 | 1,3-二甲基尿酸 | ||
| Canonical SMILES | O=C(N1C)N(C)C2=C(NC(N2)=O)C1=O | ||
| 分子式 | C7H8N4O3 | 分子量 | 196.16 |
| 溶解度 | DMSO : 5 mg/mL (25.49 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.0979 mL | 25.4894 mL | 50.9788 mL |
| 5 mM | 1.0196 mL | 5.0979 mL | 10.1958 mL |
| 10 mM | 0.5098 mL | 2.5489 mL | 5.0979 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet