1,5-Isoquinolinediol
(Synonyms: 1,5-二羟基异喹啉,NSC 65585) 目录号 : GC40468An inhibitor of poly(ADP-ribose) polymerases
Cas No.:5154-02-9
Sample solution is provided at 25 µL, 10mM.
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The poly(ADP-ribose) polymerases (PARPs) form a family of enzymes with roles in DNA repair and apoptosis. [1] 1,5-Isoquinolinediol is an inhibitor of poly(ADP-ribose) synthetase (PARP1; IC50 = 0.39 μM). [2] It has been used to study the role of PARP1 in both DNA repair and oxidant stress-induced cell death. [3][4][5] This compound can be used with cells in culture and in animals.[4][6][7]
Reference:
[1]. Davar, D., Beumer, J.H., Hamieh, L., et al. Role of PARP inhibitors in cancer biology and therapy. Current Medicinal Chemistry 19(23), 3907-3921 (2012).
[2]. Banasik, M., Komura, H., Shimoyama, M., et al. Specific inhibitors of poly(ADP-ribose) synthetase and mono(ADP-ribosyl)transferase. The Journal of Biological Chemisty 267(3), 1569-1575 (1992).
[3]. Ruscetti, T., Lehnert, B.E., Halbrook, J., et al. Stimulation of the DNA-dependent protein kinase by poly(ADP-ribose) polymerase. The Journal of Biological Chemisty 273(23), 14461-14467 (1998).
[4]. Chatterjee, P.K., Zacharowski, K., Cuzzocrea, S., et al. Inhibitors of poly (ADP-ribose) synthetase reduce renal ischemia-reperfusion injury in the anesthetized rat in vivo. The FASEB Journal 14(5), 641-651 (2000).
[5]. Bowes, J., McDonald, M.C., Piper, J., et al. Inhibitors of poly (ADP-ribose) synthetase protect rat cardiomyocytes against oxidant stress. Cardiovascular Research 41(1), 126-134 (1999).
[6]. Byun, J.Y., Kim, M.J., Eum, D.Y., et al. Reactive oxygen species-dependent activation of Bax and poly(ADP-ribose) polymerase-1 is required for mitochondrial cell death induced by triterpenoid pristimerin in human cervical cancer cells. Molecular Pharmacology 76(4), 734-744 (2009).
[7]. Kang, Y.H., Yi, M.J., Kim, M.J., et al. Caspase-independent cell death by arsenic trioxide in human cervical cancer cells: Reactive oxygen species-mediated poly(ADP-ribose) polymerase-1 activation signals apoptosis-inducing factor release from mitochondria. Cancer Research 64(24), 8960-8967 (2004).
| Cas No. | 5154-02-9 | SDF | |
| 别名 | 1,5-二羟基异喹啉,NSC 65585 | ||
| 化学名 | 5-hydroxy-1(2H)-isoquinolinone | ||
| Canonical SMILES | OC1=C2C(C(NC=C2)=O)=CC=C1 | ||
| 分子式 | C9H7NO2 | 分子量 | 161.2 |
| 溶解度 | DMSO : 37.04 mg/mL (229.83 mM; Need ultrasonic) | 储存条件 | Store at 4°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.2035 mL | 31.0174 mL | 62.0347 mL |
| 5 mM | 1.2407 mL | 6.2035 mL | 12.4069 mL |
| 10 mM | 0.6203 mL | 3.1017 mL | 6.2035 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet