1-Arachidoyl-2-hydroxy-sn-glycero-3-PA (sodium salt)
(Synonyms: LPA 20:0,PA(20:0/0:0),1-Arachidoyl LPA,1-Arachidoyl Lysophosphatidic Acid,1-Eicosanoyl-2-hydroxy-sn-glycero-3-phosphate,20:0 LPA) 目录号 : GC917851-Arachidoyl-2-hydroxy-sn-glycero-3-PA (1-arachidoyl LPA) is an agonist of lysophosphatidic acid receptor 1 (LPA1) and a glycerophospholipid containing arachidic acid at the sn-1 position.
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
1-Arachidoyl-2-hydroxy-sn-glycero-3-PA (1-arachidoyl LPA) is an agonist of lysophosphatidic acid receptor 1 (LPA1) and a glycerophospholipid containing arachidic acid at the sn-1 position.[1] It induces calcium mobilization in primary human lung fibroblasts (EC50 = 3.6 µM), which endogenously express LPA1 but not LPA2-LPA6. 1-Arachidoyl LPA (2.5 µM) also binds to the ligand-binding domain of peroxisome proliferator-activated receptor γ (PPARγ) in a cell-free assay.[2] It has been found in human urine.[3]
References:
[1].Sattikar, A., Dowling, M.R., and Rosethorne, E.M.Endogenous lysophosphatidic acid (LPA1) receptor agonists demonstrate ligand bias between calcium and ERK signalling pathways in human lung fibroblastsBr. J. Pharmacol.174(3)(2017).
[2].Tsukahara, T., Tsukahara, R., Yasuda, S., et al.Different residues mediate recognition of 1-O-oleyl-lysophosphatidic acid and rosiglitazone in the ligand binding domain of peroxisome proliferator-activated receptor γJ. Biol. Chem.281(6)3398-3407(2006).
[3].Lim, S., Byeon, S.K., Lee, J.Y., et al.Computational approach to structural identification of phospholipids using raw mass spectra from nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometryJ. Mass. Spectrom.47(8)1004-1014(2012).
| Cas No. | N/A | SDF | Download SDF |
| 别名 | LPA 20:0,PA(20:0/0:0),1-Arachidoyl LPA,1-Arachidoyl Lysophosphatidic Acid,1-Eicosanoyl-2-hydroxy-sn-glycero-3-phosphate,20:0 LPA | ||
| 分子式 | C23H46O7P•Na | 分子量 | 488.6 |
| 溶解度 | Methanol: Soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0467 mL | 10.2333 mL | 20.4666 mL |
| 5 mM | 0.4093 mL | 2.0467 mL | 4.0933 mL |
| 10 mM | 0.2047 mL | 1.0233 mL | 2.0467 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。