1-Methylinosine
(Synonyms: 1-甲基肌苷; N1-Methylinosine) 目录号 : GC65038
1-亚甲基肌苷是在次黄嘌呤环上的位置 1带有甲基取代基的肌苷。它具有作为代谢物的作用。它在功能上与肌苷有关
Cas No.:2140-73-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
HumanEndogenousMetabolite
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1-Methylinosine is a methylated purine nucleoside formed during the degradation of tRNA.1 Urinary levels of 1-methylinosine are increased in patients with acute myelomonocytic leukemia or large cell lung carcinoma. Urinary levels are also increased in patients with breast cancer and are associated with a reduced five-year survival rate.2
1.Mitchell, E.P., Evans, L., Schultz, P., et al.Modified nucleosides in human serumJ. Chromatogr.581(1)31-40(1992) 2.Sasco, A.J., Rey, F.A., Reynaud, C., et al.Breast cancer prognostic significance of some modified urinary nucleosidesCancer Lett.108(2)157-162(1996)
| Cas No. | 2140-73-0 | SDF | Download SDF |
| 别名 | 1-甲基肌苷; N1-Methylinosine | ||
| 分子式 | C11H14N4O5 | 分子量 | 282.25 |
| 溶解度 | DMSO : 20.83 mg/mL (73.80 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.543 mL | 17.7148 mL | 35.4296 mL |
| 5 mM | 0.7086 mL | 3.543 mL | 7.0859 mL |
| 10 mM | 0.3543 mL | 1.7715 mL | 3.543 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
AtTrm5a catalyses 1-methylguanosine and 1-Methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana
Nucleic Acids Res 2019 Jan 25;47(2):883-898.PMID:30508117DOI:10.1093/nar/gky1205.
Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m1G and m1I methylation was confirmed by mutant analysis, yeast complementation, m1G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a mutants were dwarfed and had short filaments, which led to reduced seed setting. Proteomics data indicated differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate were reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaired m1G and m1I methylation and led to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants.
Metabolism of 1-methyladenosine
Biochem Med Metab Biol 1987 Aug;38(1):69-73.PMID:3663399DOI:10.1016/0885-4505(87)90063-6.
1-[methyl-8-14C] Adenosine was synthesized and its metabolic fate was determined in intact rat. It was found that approximately 57% of 1-[methyl-8-14C] adenosine administered iv was excreted unchanged in the urine and 33% of the excreted radioactivity in the urine was associated with the major metabolite 1-methyl-hypoxanthine and about 4.5% was associated with 1-Methylinosine. Very little adenosine or N6-methyladenosine was formed. It is concluded that 1-methyladenosine is initially deaminated by adenosine deaminase to 1-Methylinosine which is then cleaved by nucleoside phosphorylase to 1-methylhypoxanthine.
A novel enzymatic pathway leading to 1-Methylinosine modification in Haloferax volcanii tRNA
Nucleic Acids Res 1995 Nov 11;23(21):4312-9.PMID:7501451DOI:10.1093/nar/23.21.4312.
Transfer RNAs of the extreme halophile Haloferax volcanii contain several modified nucleosides, among them 1-methylpseudouridine (m1 psi), pseudouridine (psi), 2'-0-methylcytosine (Cm) and 1-Methylinosine (m1l), present in positions 54, 55, 56 and 57 of the psi-loop, respectively. At the same positions in tRNAs from eubacteria and eukaryotes, ribothymidine (T-54), pseudouridine (psi-55), non-modified cytosine (C-56) and non-modified adenosine or guanosine (A-57 or G-57) are found in the so-called T psi-loop. Using as substrate a T7 transcript of Haloferax volcanii tRNA(Ile) devoid of modified nucleosides, the enzymatic activities of several tRNA modification enzymes, including those for m1 psi-54, psi-55, Cm-56 and m1l-57, were detected in cell extracts of H.volcanii. Here, we demonstrate that modification of A-57 into m1l-57 in H.volcanii tRNA(Ile) occurs via a two-step enzymatic process. The first step corresponds to the formation of m1A-57 catalyzed by a S-adenosylmethionine-dependent tRNA methyltransferase, followed by the deamination of the 6-amino group of the adenine moiety by a 1-methyladenosine-57 deaminase. This enzymatic pathway differs from that leading to the formation of m1l-37 in the anticodon loop of eukaryotic tRNA(Ala). In the latter case, inosine-37 formation preceeds the S-adenosylmethionine-dependent methylation of l-37 into m1l-37. Thus, enzymatic strategies for catalyzing the formation of 1-Methylinosine in tRNAs differ in organisms from distinct evolutionary kingdoms.
Archaebacterial tRNA contains 1-Methylinosine at residue 57 in T psi C-loop
Nucleic Acids Symp Ser 1982;(11):209-13.PMID:7183961doi
1-Methylinosine was isolated from unfractionated tRNAs of Sulfolobus acidocaldarius and Halobacterium volcani. The structure of 1-Methylinosine was characterized by ultraviolet absorption and mass spectral analysis. 1-Methylinosine found in these archaebacterial tRNAs was located at residue 57 in T psi C-loop, indicating that the presence of 1-Methylinosine is characteristic of archaebacteria.
Biological markers in breast carcinoma--clinical correlations with pseudouridine, N2,N2-dimethylguanosine, and 1-Methylinosine
J Surg Oncol 1980;14(3):267-73.PMID:7392649DOI:10.1002/jso.2930140313.
Urinary levels of the minor nucleosides, pseudouridine (psi),N2, N2-dimethylguanosine (m22G), and 1-Methylinosine (m1I), were investigated in patients with breast carcinoma. Elevated levels of psi were observed in 27/131 (20.6%) patients with metastatic disease, 1/14 (7.1%) preoperative patients, and 1/28 (3.6%) postoperative N+ patients. Elevated levels of m22G and M1I were observed, respectively, in 46/131 (35.1%) and 274131 (20.6%) patients with metastatic disease, 3/14 (21.4%) and 3/14 preoperative patients, and 6/28 (21.4%) and 2/28 (7.1%) postoperative N+ patients. There was no correlation between nucleoside levels and involvement of specific organ sites with metastatic disease, nor with chemotherapy response rate or time to treatment failure. During the treatment of metastatic disease there was a tendency for elevated pretherapy psi levels to decrease with attainment of a response and, if the levels subsequently rose to be associated with treatment failure. However, increasing levels of m22G and 71I occurred with both response and disease progression. These results suggest that routine measurement of the level of the urinary nucleosides would be of limited value for following the disease course in patients with breast cancer.