1-Stearoyl-2-Arachidonoyl-sn-glycero-3-PC
(Synonyms: L-α-磷脂酰胆碱-β-花生四烯酰-γ-硬脂酰,SAPC) 目录号 : GC11139A phospholipid
Cas No.:35418-59-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
1-Stearoyl-2-arachidonoyl-sn-glycero-3-PC (SAPC) is a phospholipid containing stearic acid and arachidonic acid at the sn-1 and sn-2 positions, respectively. It is a component of LDL and has been found in human stenotic aortic valves and atherosclerotic plaques.[1],[2] Levels of SAPC are increased in the subepithelial invasive region compared to the superficial region of tumor tissue samples from patients with superficial-type pharyngeal squamous cell carcinoma.[3] SAPC unilamellar vesicles have been used as substrates to quantify the activity of secretory phospholipase A2 (sPLA2) in the presence or absence of inhibitors.[4] SAPC has also been used in the formation of lipid bilayers to study the effects of lipid composition on bilayer phase transitions.[5]
Reference
[1]. Milne, G.L., Seal, J.R., Havrilla, C.M., et al. Identification and analysis of products formed from phospholipids in the free radical oxidation of human low density lipoproteins. J. Lipid Res. 46(2), 307-319 (2005).
[2]. Lehti, S., Käkelä, R., Hörkkö, S., et al. Modified lipoprotein-derived lipid particles accumulate in human stenotic aortic valves. PLoS One 8(6), e65810 (2013).
[3]. Ishikawa, S., Tateya, I., Hayasaka, T., et al. The distribution of phosphatidylcholine species in superficial-type pharyngeal carcinoma. Biomed. Res. Int. 5387913, (2017).
[4]. Schebb, N.H., Falck, D., Faber, H., et al. Fast method for monitoring phospholipase A2 activity by liquid chromatography-electrospray ionization mass spectrometry. J. Chromatogr. A 1216(27), 5249-5255 (2009).
[5]. Tada, K., Goto, M., Tamai, N., et al. Pressure effect on the bilayer phase transition of asymmetric lipids with an unsaturated acyl chain. Ann. N.Y. Acad. Sci. 1189(1), 77-85 (2010).
Cas No. | 35418-59-8 | SDF | |
别名 | L-α-磷脂酰胆碱-β-花生四烯酰-γ-硬脂酰,SAPC | ||
化学名 | 1-ocatadeconyl-2R-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glyceryl-3-phosphorylcholine | ||
Canonical SMILES | O=C(CCCCCCCCCCCCCCCCC)OC[C@@H](OC(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)=O)COP(OCC[N+](C)(C)C)([O-])=O | ||
分子式 | C46H84NO8P | 分子量 | 810.1 |
溶解度 | 30 mg/ml in Ethanol | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.2344 mL | 6.1721 mL | 12.3442 mL |
5 mM | 0.2469 mL | 1.2344 mL | 2.4688 mL |
10 mM | 0.1234 mL | 0.6172 mL | 1.2344 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。