1-Thioglycerol
(Synonyms: 1-硫代甘油) 目录号 : GC42048
A reagent used in fluorescence, spectroscopy, and microbiology techniques
Cas No.:96-27-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
1-Thioglycerol is a reagent used in fluorescence, spectroscopy, and microbiology techniques. Varying the concentration of 1-thioglycerol in the preparation of CdSe/CdS core-shell ultrasmall quantum dots (CS-USQDs) allows a precise way to control the size of the shell, with shell size increasing with increased concentration. It is used as a capping agent in the synthesis of nanocrystals analyzed via Raman and UV/Vis spectroscopy. Biologically, 1-thioglycerol stimulates porphyrin synthesis and increases glutamyl-tRNA reductase activity in E. coli grown in an aerobic environment. It was previously a component in hair permanents used to produce waves or curls.
| Cas No. | 96-27-5 | SDF | |
| 别名 | 1-硫代甘油 | ||
| Canonical SMILES | SCC(O)CO | ||
| 分子式 | C3H8O2S | 分子量 | 108.2 |
| 溶解度 | DMF: 20 mg/ml,DMSO: 10 mg/ml,Ethanol: 30 mg/ml,Ethanol:PBS (pH 7.2)(1:2): 0.25 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 9.2421 mL | 46.2107 mL | 92.4214 mL |
| 5 mM | 1.8484 mL | 9.2421 mL | 18.4843 mL |
| 10 mM | 0.9242 mL | 4.6211 mL | 9.2421 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
1-Thioglycerol: inhibitor of glycerol kinase activity in vitro and in situ
Life Sci 1986 Oct 20;39(16):1417-24.PMID:3022087DOI:10.1016/0024-3205(86)90545-x.
The infantile form of glycerol kinase (GK) deficiency (McKusick No. 30703) (1) is characterized by adrenal cortical insufficiency, adrenal hypoplasia and developmental delay. The underlying biochemical mechanism(s) responsible for the observed clinical presentations are undetermined. Pursuant to our examination of the molecular pathogenesis of this enzyme deficiency, we have endeavored to develop a model for this disorder. 1-Thioglycerol (1-TG) was investigated as a potential GK inhibitor in adrenal gland, an organ consistently affected, and in cultured fibroblasts, available from affected individuals. In 105,000 g bovine adrenal supernatant the Ki for 1-TG was 1.9 mM. In human fibroblast 105,000 g supernatant, the Ki for 1-TG was 3.4 mM. In both tissues the inhibition was purely competitive with respect to glycerol. Using incorporation of [14C(U)]-glycerol into protein as an index of GK activity in situ in human skin fibroblasts, GK deficient fibroblasts incorporate less than 10% of that observed in normal fibroblasts. Addition of 1-TG to normal fibroblasts resulted in inhibited incorporation rates. The specificity of these effects in situ was examined. Our findings indicate that 1-TG may be a suitable inhibitor of GK activity for the development of a model for glycerol kinase deficiency.
Thiol-functionalized copolymeric polyesters by lipase-catalyzed esterification and transesterification of 1,12-dodecanedioic acid and its diethyl ester, respectively, with 1-Thioglycerol
Biotechnol Lett 2010 Oct;32(10):1463-71.PMID:20495948DOI:10.1007/s10529-010-0311-z.
Copolymeric polyoxoesters containing branched-chain methylenethiol functions, i.e., poly(1,12-dodecanedioic acid-co-1-thioglycerol) and poly(diethyl 1,12-dodecanedioate-co-1-thioglycerol), were formed by lipase-catalyzed polyesterification and polytransesterification of 1,12-dodecanedioic acid and diethyl 1,12-dodecanedioate, respectively, with 1-Thioglycerol (3-mercaptopropane-1,2-diol) using immobilized lipase B from Candida antarctica (Novozym 435) in vacuo without drying agent in the reaction mixture. After 360-480 h, both polyoxoesters were purified by extraction from the reaction mixtures followed by solvent fractionation. The precipitate of poly(1,12-dodecanedioic acid-co-1-thioglycerol) demonstrated a M(W) of ~170,000 Da, whereas a M(W) of ~7,100 Da only was found for poly(diethyl 1,12-dodecanedioate-co-1-thioglycerol). Both polycondensates were analyzed by GPC/SEC, alkali-catalyzed transmethylation, NMR- and FTIR-spectrometry.
Shell thickness modulation in ultrasmall CdSe/CdS(x)Se(1-x)/CdS core/shell quantum dots via 1-Thioglycerol
ACS Nano 2014 Feb 25;8(2):1913-22.PMID:24460449DOI:10.1021/nn406478f.
In this study, we report on the synthesis of CdSe/CdS core-shell ultrasmall quantum dots (CS-USQDs) using an aqueous-based wet chemistry protocol. The proposed chemical route uses increasing concentration of 1-Thioglycerol to grow the CdS shell on top of the as-precipitated CdSe core in a controllable way. We found that lower concentration of 1-Thioglycerol (3 mmol) added into the reaction medium limits the growth of the CdSe core, and higher and increasing concentration (5-11 mmol) of 1-Thioglycerol promotes the growth of CdS shell on top of the CdSe core in a very controllable way, with an increase from 0.50 to 1.25 nm in shell thickness. The growth of CS-USQDs of CdSe/CdS was confirmed by using different experimental techniques, such as optical absorption (OA) spectroscopy, fluorescence spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and Raman spectroscopy. Data collected from OA were used to obtain the average values of the CdSe core diameter, whereas Raman data were used to assess the average values of the CdSe core diameter and CdS shell thicknesses.
The effect of surface attachment on ligand binding: studying the association of Mg2+, Ca2+ and Sr2+ by 1-Thioglycerol and 1,4-dithiothreitol monolayers
Phys Chem Chem Phys 2006 Jan 7;8(1):158-64.PMID:16482256DOI:10.1039/b511285k.
The difference in the heterogeneous binding of Mg(2+), Ca(2+) and Sr(2+) ions by 1-Thioglycerol (TG) and 1,4-dithiothreitol (DTT) spontaneously adsorbed monolayers on Au has been studied following the changes in the double layer capacity. A mathematical treatment, based on calculating the electrochemical potential difference at the monolayer-electrolyte interface, has followed our recent work which dealt with the acid-base equilibrium at the interface as a means of calculating the pK of ionizable SAMs and their binding with Cd(2+). Experimentally, spontaneously adsorbed monolayers of TG and DTT were assembled on Au surfaces and studied by impedance spectroscopy and alternating current voltammetry (ACV). The capacity was measured for each of the modified surfaces at increasing concentrations of the divalent metal ions separately. The goal of this study has been to examine the effect of metal ion binding by similar ligands that are differently attached onto the surface. TG and DTT monolayers differ in their flexibility, which is a result of their attachment to the surface through one and two arms, respectively. The general trend of the apparent heterogeneous association constants of the divalent metal ions, which were calculated from the capacity measurements, was substantially different from the classical Irving-Williams series that is applicable to homogeneous systems. This difference could be nicely explained by the reduction of the degree of freedom and flexibility of the attached ligands.
Thioglycerol-capped Mn-doped ZnS quantum dot bioconjugates as efficient two-photon fluorescent nano-probes for bioimaging
J Mater Chem B 2013 Feb 7;1(5):698-706.PMID:32260775DOI:10.1039/c2tb00247g.
Water-dispersible 1-Thioglycerol (TG)-capped Mn-doped ZnS quantum dots were prepared in aqueous solution using the nucleation-doping strategy. Using 4% Mn relative to Zn and a Zn(OAc)2/Na2S ratio of 0.9, Mn:ZnS nanocrystals with an average diameter of 3.9 ± 0.5 nm, with pure Mn2+-related photoluminescence (PL) at 585 nm, and with a PL quantum yield of 13.2% were obtained. Transmission electron microscopy, X-ray powder diffraction, electron spin resonance, X-ray photoelectron spectroscopy, UV-visible spectroscopy and spectrofluorometry have been used to characterize the crystal structure, the doping status, and the optical properties of the doped-dots. Folic acid (FA) was linked to TG-capped Mn:ZnS nanocrystals to produce Mn:ZnS@TG-FA nanobioconjugates that were used for targeted in vitro delivery to a human cancer cell line. Folate receptor mediated cellular uptake of FA-functionalized dots is proven via confocal and two-photon imaging.