10Panx
(Synonyms: 10Panx) 目录号 : GC13863
A peptide inhibitor of PANX1
Cas No.:955091-53-9
Sample solution is provided at 25 µL, 10mM.
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10Panx is a mimetic inhibitory peptide of panx1, which is a mammalian protein ectopically expressed to function as a hemi-channel, that readily and reversibly inhibits the panx1 currents. 10Panx has attracted increasing attention as it allows scientists readily and reliably to examine inhibition of panx1 in cells where efficient and consistent delivery of siRNA is highly difficult. According to results of multiple previous studies, 10Panx selectively inhibits P2X7R-induced dye uptake without altering other aspects of R2X7R activation and ATP-evoked membrane currents, constitutive dye-uptake and hemichannel-like currents that are induced by the overexpression of panx1, and ATP-mediated IL-1β release.
Reference
[1].Pablo Pelegrin and Annmarie Surprenant. Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X7 receptor. The EMBO Journal (2006) 25, 5071-5082
| Cell experiment: [1] | |
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Cell lines |
Human red blood cells (RBCs) |
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Preparation method |
The solubility of this peptide in sterile water is >10 mM. Stock solution should be splited and stored at -80°C for several months. |
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Reaction Conditions |
200 μM, |
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Applications |
10Panx reduced ATP release in response to lowered pO2 by 90.9±15.5%. It did not reduce ILO-induced ATP release. |
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Animal models |
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Dosage form |
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Applications |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Sridharan M, Adderley S, Bowles E, et al. Pannexin 1 (PANX1) is a Conduit for ATP Released from Human Red Blood Cells (RBCs) Exposed to Low Oxygen Tension. The FASEB Journal, 2010. | |
| Cas No. | 955091-53-9 | SDF | |
| 别名 | 10Panx | ||
| Canonical SMILES | CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)O)NC(=O)C(CC2=CC=CC=C2)NC(=O)C(C)NC(=O)C(C)NC(=O)C(CCC(=O)N)NC(=O)C(CCCN=C(N)N)NC(=O)C(CC3=CNC4=CC=CC=C43)N | ||
| 分子式 | C58H79N15O16 | 分子量 | 1242.37 |
| 溶解度 | ≥ 124.2mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.8049 mL | 4.0246 mL | 8.0491 mL |
| 5 mM | 0.161 mL | 0.8049 mL | 1.6098 mL |
| 10 mM | 0.0805 mL | 0.4025 mL | 0.8049 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet