11-keto Testosterone
(Synonyms: 11-酮睾酮,11-Oxotestosterone, 11-酮基睾酮) 目录号 : GC18192
11-keto Testosterone（11-酮基睾酮）是一种氧化形式的睾酮，是一种非芳香化的活性雄激素。
Cas No.:564-35-2
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
11-keto Testosterone is an oxidized form of testosterone and a non-aromatizable active androgen[1, 2]. 11-keto Testosterone is the major androgen in fish[3]. 11-keto Testosterone can promote the degeneration of oocytes, the proliferation of spermatocytes, and the formation of sperm in grouper[4].
In vitro, 11-keto Testosterone (10, 100μM) treatment of Sterlet liver explants and ovarian explants for 5 days significantly increased the concentrations of testosterone and 17β-estradiol in the culture medium of liver explants and ovarian explants, and the concentration of vitellogenin in the culture medium of liver explants also increased significantly[5].
In vivo, silicone rubber strips coated with 11-keto Testosterone (5, 25mg/kg/day) were implanted into pre-pregnant female Sterlet for 30 days and promoted ovarian development in a dose-dependent manner, but no ovarian masculinization was found[5].
References:
[1] Stárka L, Dušková M, Vítků J. 11-Keto-testosterone and other androgens of adrenal origin[J]. Physiological Research, 2020, 69(Suppl 2): S187.
[2] Felício A A, Crago J, Maryoung L A, et al. Effects of alkylphenols on the biotransformation of diuron and enzymes involved in the synthesis and clearance of sex steroids in juvenile male tilapia (Oreochromus mossambica)[J]. Aquatic Toxicology, 2016, 180: 345-352.
[3] Loggie J W. A test of the effects of androgens on immunity: no relationship between 11-keto testosterone and immune performance in bluegill sunfish (Lepomis macrochirus)[D]. The University of Western Ontario (Canada), 2016.
[4] Lee C H, Hur S W, Song Y B, et al. Effect of 11-ketotestosterone (11-KT) on gonadal sex reversal and spermatogenesis of honeycomb grouper Epinephelus merra[J]. Development and Reproduction, 2010, 14(1): 1-5.
[5] Wang W, Zhu H, Tian Z, et al. Effects of 11-Ketotestosterone on development of the previtellogenic ovary in the sterlet, Acipenser Ruthenus. Front Endocrinol (lausanne) 11: 115[J]. 2020.
11-keto Testosterone（11-酮基睾酮）是一种氧化形式的睾酮，是一种非芳香化的活性雄激素[1, 2]。11-keto Testosterone在鱼类中是主要的雄激素[3]。11-keto Testosterone能促进石斑鱼卵母细胞的变性、精母细胞的增殖和精子的形成[4]。
在体外，11-keto Testosterone（10, 100μM）处理Sterlet（小体鲟）的肝外植体和卵巢外植体5天，显著增加了肝外植体和卵巢外植体培养基中的睾酮和17β-雌二醇的浓度，肝外植体培养基中的卵黄蛋白原浓度也显著增加[5]。
在体内，11-keto Testosterone（5, 25mg/kg/day）包埋的硅橡胶条植入孕前期雌性Sterlet体内30天，以剂量依赖性方式促进了卵巢发育，未发现卵巢雄性化[5]。
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.3069 mL | 16.5344 mL | 33.0688 mL |
| 5 mM | 0.6614 mL | 3.3069 mL | 6.6138 mL |
| 10 mM | 0.3307 mL | 1.6534 mL | 3.3069 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。