11beta-Hydroxyprogesterone (11β-Hydroxyprogesterone)
(Synonyms: 11β-羟孕酮; 11β-Hydroxyprogesterone) 目录号 : GC34016
A metabolite of progesterone
Cas No.:600-57-7
Sample solution is provided at 25 µL, 10mM.
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11β-Hydroxyprogesterone (11β-OHP) is a metabolite of progesterone .1 It is an agonist of the mineralocorticoid receptor (MR) that induces MR transactivation in a reporter assay (EC50 = 0.05 ?M for COS-7 cells expressing the human receptor).2 It increases sodium absorption in mpkCCDc14 murine renal cortical collecting duct cells. 11β-OHP is an inhibitor of 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) and 11β-HSD1 (IC50s = 0.140 and 1 ?M, respectively).3 It increases blood pressure in rats when administered subcutaneously at a rate of 10 ?g/hour.4 Serum levels of 11β-OHP are increased in patients with 21-hydroxylase deficiency, a type of congenital adrenal hyperplasia (CAH), characterized by masculinized genitals in female infants, irregular menses in adult females, and accelerated bone age in adolescent males.5
1.van Rooyen, D., Gent, R., Barnard, L., et al.The in vitro metabolism of 11β-hydroxyprogesterone and 11-ketoprogesterone to 11-ketodihydrotestosterone in the backdoor pathwayJ. Steroid Biochem. Mol. Biol.178203-212(2018) 2.Rafestin-Oblin, M.-E., Fagart, J., Souque, A., et al.11β-hydroxyprogesterone acts as a mineralocorticoid agonist in stimulating Na+ absorption in mammalian principal cortical collecting duct cellsMol. Pharmacol.62(6)1306-1313(2002) 3.Souness, G.W., Latif, S.A., Laurenzo, J.L., et al.11α- and 11β-hydroxyprogesterone, potent inhibitors of 11β-hydroxysteroid dehydrogenase (isoforms 1 and 2), confer marked mineralocorticoid activity on corticosterone in the ADX ratEndocrinology136(4)1809-1812(1995) 4.Souness, G.W., and Morris, D.J.11α- and 11β-hydroxyprogesterone, potent inhibitors of 11β-hydroxysteroid dehydrogenase, possess hypertensinogenic activity in the ratHypertension27(3 Pt 1)421-425(1996) 5.Turcu, A.F., Rege, J., Chomic, R., et al.Profiles of 21-carbon steroids in 21-hydroxylase deficiencyJ. Clin. Endocrinol. Metab.100(6)2283-2290(2015)
Animal experiment: | Rats: 11α- and 11β-OHP are dissolved in propylene glycol (100%) and infused at 3 and 10 μg/h, respectively, for 14 days. Control rats received vehicle only. BP is measured the day before pumps were implanted and on days 3, 7, 10, and 14 after implantation. Indirect systolic BPs are measured with a modified tail-cuff method[2]. |
References: [1]. Rafestin-Oblin ME, et al. 11beta-hydroxyprogesterone acts as a mineralocorticoid agonist in stimulating Na+ absorption in mammalian principal cortical collecting duct cells. Mol Pharmacol. 2002 Dec;62(6):1306-13. | |
| Cas No. | 600-57-7 | SDF | |
| 别名 | 11β-羟孕酮; 11β-Hydroxyprogesterone | ||
| Canonical SMILES | C[C@@]12[C@]3([H])[C@](CCC1=CC(CC2)=O)([H])[C@@]4([H])[C@@](C)([C@@H](C(C)=O)CC4)C[C@@H]3O | ||
| 分子式 | C21H30O3 | 分子量 | 330.46 |
| 溶解度 | DMSO : 50 mg/mL (151.30 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0261 mL | 15.1304 mL | 30.2608 mL |
| 5 mM | 0.6052 mL | 3.0261 mL | 6.0522 mL |
| 10 mM | 0.3026 mL | 1.513 mL | 3.0261 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet