(±)12(13)-EpOME MaxSpec® Standard
(Synonyms: (±)12,13-EODE, Isoleukotoxin, (±)-Vernolic Acid) 目录号 : GC48807
A quantitative analytical standard guaranteed to meet MaxSpec identity, purity, stability, and concentration specifications
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
(±)12(13)-EpOME is the 12,13-cis epoxide form of linoleic acid .1,2 It is formed primarily via linoleic acid metabolism by the cytochrome P450 (CYP) isoforms CYP2J2, CYP2C8, and CYP2C9, however, CYP1A1 can contribute to (±)12(13)-EpOME production when pharmacologically induced.2 (±)12(13)-EpOME (500 µM) induces mitochondrial dysfunction and cell death in renal proximal tubule epithelial cells.
(±)12(13)-EpOME MaxSpec® standard is a quantitative grade standard of (±)12(13)-EpOME that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This (±)12(13)-EpOME MaxSpec® standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.
1.Hayakawa, M., Sugiyama, S., Takamura, T., et al.Neutrophils biosynthesize leukotoxin, 9,10-epoxy-12-octadecenoateBiochem. Biophys. Res. Commun.137(1)424-430(1986) 2.Hildreth, K., Kodani, S.D., Hammock, B.D., et al.Cytochrome P450-derived linoleic acid metabolites EpOMEs and DiHOMEs: A review of recent studiesJ. Nutr. Biochem.19108484(2020)
| Cas No. | SDF | ||
| 别名 | (±)12,13-EODE, Isoleukotoxin, (±)-Vernolic Acid | ||
| Canonical SMILES | CCCCC[C@@H]1[C@@H](O1)C/C=C\CCCCCCCC(O)=O | ||
| 分子式 | C18H32O3 | 分子量 | 296.5 |
| 溶解度 | 储存条件 | -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.3727 mL | 16.8634 mL | 33.7268 mL |
| 5 mM | 0.6745 mL | 3.3727 mL | 6.7454 mL |
| 10 mM | 0.3373 mL | 1.6863 mL | 3.3727 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。