2',3'-Dideoxyguanosine-5'-O-triphosphate (sodium salt)
(Synonyms: ddGTP) 目录号 : GC90805一种反转录酶抑制剂
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2',3'-Dideoxyguanosine-5'-O-triphosphate (ddGTP) is a reverse transcriptase inhibitor (Kis = 13 and 6.7 nM for the visna virus and HIV-1 enzymes, respectively).1,2 ddGTP has been used in the synthesis of oligonucleotides containing a terminal ddG that are resistant to cleavage by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exonuclease.3 ddGTP has also been used to terminate chain extension produced by the Taq polymerase in PCR assays.4
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.7268 mL | 8.6341 mL | 17.2682 mL |
5 mM | 0.3454 mL | 1.7268 mL | 3.4536 mL |
10 mM | 0.1727 mL | 0.8634 mL | 1.7268 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。