2,4-D Butyl ester
(Synonyms: 2,4-滴丁酯) 目录号 : GC60460
2,4-DButylester是一种有机苯氧基除草剂，用于防治木质阔叶杂草。2,4-DButylester通过模仿天然植物生长激素导致植物生长太快而无法生存，从而产生除草作用。
Cas No.:94-80-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
2,4-D Butyl ester is an organic phenoxy herbicide and used to control woody broad-leaf weeds. 2,4-D butyl ester produces its herbicidal effect by mimicking natural plant growth hormones causing plants to grow too rapidly to survive[1][2].
[1]. Schulze GE, et, al. Formulation and food deprivation affects 2,4-D neurobehavioral toxicity in rats. Neurotoxicol Teratol. Sep-Oct 1987;9(5):363-7. [2]. Xiao L, et, al. Isolation and Characterization of 2,4-D Butyl Ester Degrading Acinetobacter sp. ZX02 from a Chinese Ginger Cultivated Soil. J Agric Food Chem. 2017 Aug 30;65(34):7345-7351.
| Cas No. | 94-80-4 | SDF | |
| 别名 | 2,4-滴丁酯 | ||
| Canonical SMILES | O=C(OCCCC)COC1=CC=C(Cl)C=C1Cl | ||
| 分子式 | C12H14Cl2O3 | 分子量 | 277.14 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6083 mL | 18.0414 mL | 36.0828 mL |
| 5 mM | 0.7217 mL | 3.6083 mL | 7.2166 mL |
| 10 mM | 0.3608 mL | 1.8041 mL | 3.6083 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Characterization of a novel lipase from Bacillus licheniformis NCU CS-5 for applications in detergent industry and biodegradation of 2,4-D Butyl ester
Int J Biol Macromol 2021 Apr 15;176:126-136.PMID:33548313DOI:10.1016/j.ijbiomac.2021.01.214.
Enzymatic degradation has become the most promising approach to degrading organic ester compounds. In this study, Bacillus licheniformis NCU CS-5 was isolated from the spoilage of Cinnamomum camphora seed kernel, and its extracellular lipase was purified, with a specific activity of 192.98 U/mg. The lipase was found to be a trimeric protein as it showed a single band of 27 kDa in SDS-PAGE and 81 kDa in Native-PAGE. It was active in a wide range of temperatures (5-55 °C) and pH values (6.0-9.0), and the optimal temperature and pH value were 40 °C and 8.0, respectively. The enzyme was active in the presence of various organic solvents, metal ions, inhibitors and surfactants. Both crude and purified lipase retained more than 80% activity after 5 h in the presence of commercial detergents, suggesting its great application potential in detergent industry. The highest activity was found to be towards medium- and long-chain fatty acids (C6-C18). Peptide mass spectrometric analysis of the purified lipase showed similarity to the lipase family of B. licheniformis. Furthermore, it degraded more than 90% 2,4-D Butyl ester to its hydrolysate 2,4-D within 24 h, indicating that the novel lipase may be applied to degrade organic ester pesticides.
Isolation and Characterization of 2,4-D Butyl ester Degrading Acinetobacter sp. ZX02 from a Chinese Ginger Cultivated Soil
J Agric Food Chem 2017 Aug 30;65(34):7345-7351.PMID:28771369DOI:10.1021/acs.jafc.7b02140.
Strain ZX02 was isolated from Chinese ginger cultivated soil contaminated with various pesticides, which could utilize 2,4-dichlorophenoxyacetic acid butyl ester (2,4-D Butyl ester) as the sole carbon source. On the basis of the sequence analysis of 16S rRNA gene as well as the morphological, biochemical, and physiological characteristics of strain ZX02, the organism belonged to Gram-negative bacterium and was identified as Acinetobacter sp. ZX02. The strain ZX02 showed a remarkable performance in 2,4-D Butyl ester degradation (100% removal in <96 h) in pure culture. Strain ZX02 was sensitive to tetracycline and resistant to amoxicillin and chloramphenicol in an antibiotic sensitivity test. The curing study indicates that the gene for degradation of 2,4-D Butyl ester was encoded on a single plasmid of 23 kb. The gene encoding resistance to polymixin B sulfate was also located on this plasmid. On the basis of its greater biodegradation activity, this bacterium is a potential candidate as a bioremediation agent in soils contaminated with 2,4-D Butyl ester.
The metabolic fate of 2,4-dichlorophenoxyacetic acid-n-butyl ester in the Wistar rat
Arch Toxicol 1985 Sep;57(4):231-6.PMID:4091647DOI:10.1007/BF00324783.
The metabolic fate of 2,4-dichlorophenoxyacetic acid (2,4-D)-n-butyl ester in rats has not been extensively studied. Upon subcutaneous administration of a 100 mg/kg dose of 2,4-D Butyl ester to four male Wistar rats, urine samples were analyzed by three analytical techniques for the presence of the butyl ester and metabolites. Thin layer chromatography (TLC), gas chromatography using an electron capture detector (GC-ECD), and gas chromatography-mass spectroscopy (GC-MS) were the techniques employed. 2,4-D Butyl ester was rapidly hydrolyzed in the body to form 2,4-D acid. Ninety-five percent of the administered dose was excreted into the urine as the free acid within 48 h of injection, while only a small fraction (5%) was excreted over an additional 48 h. No amino acid conjugates or the parent 2,4-D Butyl ester could be detected in the urine of treated rats. A minor metabolite (less than or equal to 2% of dose) was detected by GC-MS analysis of urine samples. This compound appears to be a side chain metabolite of the 2,4-D Butyl ester. Some chemical properties of the metabolite were characterized, and a 2,4-D hydroxyethyl ester structure proposed. The mechanism of this minor metabolic formation remains unknown.
Formation and contamination of PCDD/Fs, PCBs, PeCBz, HxCBz and polychlorophenols in the production of 2,4-D products
Chemosphere 2013 Jul;92(3):304-8.PMID:23601123DOI:10.1016/j.chemosphere.2013.03.031.
The concentrations and profiles of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs), pentachlorobenzene (PeCBz), hexachlorobenzene (HxCBz) and polychlorophenols in 2,4-D were investigated in this study. Two 2,4-D acid and three 2,4-D Butyl ester enterprises were selected as typical 2,4-D producers. The total concentrations of 2,3,7,8-PCDD/Fs in the 2,4-D samples ranged from 355 to 35080ngkg(-1) and the corresponding TEQ values were in the range of 13.4 and 694.6ng WHO-TEQkg(-1). The concentrations of total PCBs in the 2,4-D were in the range of 16.1 and 8023ngkg(-1), and the WHO-TEQ values of the PCBs were between 0.057 and 108.3ng WHO-TEQkg(-1), while total PCBs were between 1486 and 47342ngkg(-1). The average emission factors were 414.4μg WHO-TEQt(-1) for PCDD/Fs and 21.9μg WHO-TEQt(-1) for PCBs. The polychlorobenzenes and polychlorophenols impurities may play a key role in the PCBs and PCDD/Fs formation. The impurities of PCDD/Fs and PCBs in 2,4-D may increase the risk for the human and environmental health.
Chick brain hypomyelination produced by 2,4-dichlorophenoxyacetic butyl ester treatment
Neurotoxicology 1985 Fall;6(3):133-7.PMID:2995884doi
Fertilized hens' eggs were externally treated with 2,4-dichlorophenoxyacetic (2,4-D) butyl ester (2.4 mg/egg) before starting incubation. The specific activity of 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase) was diminished in the whole brain of animals born from treated eggs, as compared with controls. Myelin was isolated by ultracentrifugation from the central nervous system. Treatment with 2,4-D Butyl ester produced a 65% reduction. There were no statistically significant differences in myelin phospholipids, cerebrosides, sulphatides and gangliosides between control and treated animals. Cholesterol was diminished by 12% in the treated group, while cholesterol esters were not detectable in either myelin fraction. Total myelin proteins were also decreased by 40%, without variations in their pattern. There were changes in the following ratios in the myelin of the treated group: lipid/protein, cholesterol/phospholipids, cholesterol/protein, phospholipids/protein. Collectively these findings indicate that 2,4-D Butyl ester produced hypomyelination.