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2',5'-dideoxy Adenosine Sale

(Synonyms: 2ʹ,5ʹ-dd-Ado,NSC 95943) 目录号 : GC17544

An adenylate cyclase inhibitor

2',5'-dideoxy Adenosine Chemical Structure

Cas No.:6698-26-6

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1mg
¥244.00
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5mg
¥941.00
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10mg
¥1,485.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

2',5'-dideoxy Adenosine, a nucleoside analog, is one of the first identified cell-permeable, P-site inhibitors of adenylate cyclase [1].

Adenylyl cyclase is an enzyme with key regulatory roles in almost all cells. Adenylyl cyclases have been involved in catalyzing the conversion of ATP to cAMP) and pyrophosphate [2]. 2',5'-dideoxy Adenosine inhibits forskolin-induced activation of a cAMP-dependent reporter gene in HEK293 cells with an IC50 value of 33 μM.

In vitro: In HEK293 cells expressing a cAMP response element (CRE) reporter gene, 2',5'-dideoxy Adenosine (ddAd) effectively and potently inhibited the activity of adenylate cyclase (AC). The ddAd effectively inhibited the effect of forskolin, an AC activator, with IC50 value of 33 μM [1]. Pretreatment with graded concentrations of ddAd effectively inhibited PACAP-induced reporter gene activation with IC50 value of ~35 μM [1]. The ddAd inhibited forskolin-induced Elk-1 transactivation with an IC50 of 10 μM. The ddAd at concentrations greater than 500 mM failed to completely inhibit PACAP-induced cAMP elevation[1].

References:
[1] Emery A C, Eiden M V, Eiden L E.  A new site and mechanism of action for the widely used adenylate cyclase inhibitor SQ22, 536[J]. Molecular pharmacology, 2013, 83(1): 95-105.
[2] Gilman A G.  G proteins and dual control of adenylate cyclase[J]. Cell, 1984, 36(3): 577-579.

Chemical Properties

Cas No. 6698-26-6 SDF
别名 2ʹ,5ʹ-dd-Ado,NSC 95943
化学名 2',5'-dideoxy-adenosine
Canonical SMILES C[C@@H]1[C@@H](O)C[C@H](N2C=NC3=C2N=CN=C3N)O1
分子式 C10H13N5O2 分子量 235.2
溶解度 ≤20mg/ml in DMSO;5mg/ml in dimethyl formamide 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
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1 mg 5 mg 10 mg
1 mM 4.2517 mL 21.2585 mL 42.517 mL
5 mM 0.8503 mL 4.2517 mL 8.5034 mL
10 mM 0.4252 mL 2.1259 mL 4.2517 mL
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