2-Ketoglutaric acid
(Synonyms: α-酮戊二酸; Alpha-Ketoglutaric acid) 目录号 : GC30136
An intermediate in the citric acid cycle
Cas No.:328-50-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
α-Ketoglutaric acid is an α-keto acid and a rate-determining metabolic intermediate in the citric acid cycle in its conjugate base form, α-ketoglutarate.1,2 It is formed via oxidative decarboxylation of isocitrate by isocitrate dehydrogenase (IDH), oxidative deamination of glutamate by glutamate dehydrogenase, or by pyridoxal phosphate-dependent transamination.2 α-Ketoglutaric acid is decarboxylated to succinyl-CoA by α-ketoglutarate dehydrogenase, a rate limiting step in the citric acid cycle. It is a precursor of glutamine and glutamate, energy sources for enterocytes and various immune cells, and an antioxidant with roles in immune homeostasis, aging, protein synthesis, and bone development.2,3
1.Krebs, H.A., Salvin, E., and Johnson, W.A.The formation of citric and α-ketoglutaric acids in the mammalian bodyBiochem. J.32(1)113-117(1938) 2.Wu, N., Yang, M., Gaur, U., et al.Alpha-ketoglutarate: Physiological functions and applicationsBiomol. Ther. (Seoul)24(1)1-8(2016) 3.Liu, S., He, L., and Yao, K.The antioxidative function of alpha-ketoglutarate and its applicationsBiomed. Res. Int.3408467(2018)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 6.8446 mL | 34.2231 mL | 68.4463 mL |
| 5 mM | 1.3689 mL | 6.8446 mL | 13.6893 mL |
| 10 mM | 0.6845 mL | 3.4223 mL | 6.8446 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。