24(S)-hydroxy Cholesterol
(Synonyms: (3Β,24S)-胆甾-5-烯-3,24-二醇,Cerebrosterol) 目录号 : GC18076
An agonist of LXR receptors
Cas No.:474-73-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
24S-hydroxycholesterol (24S-OH-Chol) is an enzymatically oxidized product of cholesterol mainly synthesized in the brain, involved in the pathogenesis of Alzheimer disease (AD) [1].
24(S)-hydroxy Cholesterol has been generated by the action of enzyme cholesterol 24(S)-hydroxylase (CYP46) on cholesterol in the brain. 24(S)-hydroxy Cholesterol can diffuse across the blood-brain barrier to the systemic circulation where it modulates cell signaling, which could be used for further sterol biosynthesis, or be metabolized in the liver. In brain, disturbances of cholesterol homeostasis have been associated with severe neurological diseases and play a dominant role in the development of Alzheimer disease (AD).
In AD patients, the levels of 24(S)-hydroxycholesterol have been increased in cerebrospinal fluid [1]. 24(S)-hydroxycholesterol is able to induce the expression of ABCA1, ABCG1, and apoE in astrocytes and to elevate apoE-mediated cholesterol efflux in astrocytoma but not in neuroblastoma cells [1]. The concentration of 24(S)-hydroxycholesterol in AD and non-Alzheimer demented patients was modestly but significantly higher than in healthy volunteers and in depressed patients. Plasma 24(S)-hydroxycholesterol levels negatively correlated with the severity of dementia. 24(S)-hydroxycholesterol plasma levels may potentially be used as an early biochemical marker for an altered cholesterol homeostasis in the central nervous system [2]. 24(S)-hydroxycholesterol is a natural ligand of the liver X receptors (LXR), central players in the regulation of cholesterol metabolism. 24(S)-hydroxycholesterol potently activated LXRα and LXRβ nuclear receptors with the EC50 values of 4 and 3 μM, respectively, causing upregulation of cholesterol-lowering genes.
References:
[1] Abildayeva K, Jansen P J, Hirsch-Reinshagen V, et al. 24 (S)-hydroxycholesterol participates in a liver X receptor-controlled pathway in astrocytes that regulates apolipoprotein E-mediated cholesterol efflux[J]. Journal of Biological Chemistry, 2006, 281(18): 12799-12808.
[2] Lütjohann D, Papassotiropoulos A, Bjrkhem I, et al. Plasma 24S-hydroxycholesterol (cerebrosterol) is increased in Alzheimer and vascular demented patients[J]. Journal of Lipid Research, 2000, 41(2): 195-198.
[3] Janowski, B. A.,Grogan, M.J.,Jones, S.A., et al. Structural requirements of ligands for the oxysterol liver X receptors LXRα and LXRβ. Proceedings of the National Academy of Sciences of the United States of America 96(1), 266-271 (1999).
Cas No. | 474-73-7 | SDF | |
别名 | (3Β,24S)-胆甾-5-烯-3,24-二醇,Cerebrosterol | ||
化学名 | cholest-5-ene-3β,24S-diol | ||
Canonical SMILES | O[C@@H]1CC2=CC[C@]3([H])[C@@](CC[C@]4([C@@]3([H])CC[C@]4([H])[C@@H](CC[C@H](O)C(C)C)C)C)([H])[C@](C)2CC1 | ||
分子式 | C27H46O2 | 分子量 | 402.7 |
溶解度 | ≤20mg/ml in ethanol; 100μg/ml in DMSO; 2mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
![]() |
1 mg | 5 mg | 10 mg |
1 mM | 2.4832 mL | 12.4162 mL | 24.8324 mL |
5 mM | 0.4966 mL | 2.4832 mL | 4.9665 mL |
10 mM | 0.2483 mL | 1.2416 mL | 2.4832 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。