3-Bromotyrosine-13C9,15N (trifluoroacetate salt)
(Synonyms: 3-bromo-Tyr-13C9,15N) 目录号 : GC42252
An internal standard for the quantification of 3-bromotyrosine
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
3-Bromotyrosine-13C9,15N is intended for use as an internal standard for the quantification of 3-bromotyrosine by GC- or LC-MS. 3-Bromotyrosine is a product of protein oxidation found after activation of eosinophils during an allergic response. It has been used as a marker of eosinophil peroxidase-induced protein oxidation in vitro and in vivo. In addition to its production in eosinophils, 3-bromotyrosine is found at 5-fold higher levels in peritoneal exudate from mice infected with K. pneumoniae compared with uninfected animals, suggesting neutrophils release higher levels of oxidized products during inflammation. In a human study, 3-bromotyrosine was increased in allergen-challenged lung samples from subjects with allergen-induced asthma.
| Cas No. | SDF | ||
| 别名 | 3-bromo-Tyr-13C9,15N | ||
| Canonical SMILES | Br[13C]1=[13C](O)[13CH]=[13CH][13C]([13CH2][13C@H]([15NH2])[13C](O)=O)=[13CH]1.OC(C(F)(F)F)=O | ||
| 分子式 | [13C9]H10Br[15N]O3•CF3COOH | 分子量 | 384 |
| 溶解度 | DMF: 30 mg/mL,DMSO: 30 mg/mL,Ethanol: 30 mg/mL,PBS (pH 7.2): 10 mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.6042 mL | 13.0208 mL | 26.0417 mL |
| 5 mM | 0.5208 mL | 2.6042 mL | 5.2083 mL |
| 10 mM | 0.2604 mL | 1.3021 mL | 2.6042 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。