3-Deazaadenosine hydrochloride
(Synonyms: c3Ado, deaza-Ado, 3-DZA) 目录号 : GC33842
An inhibitor of S-adenosylhomocysteine
Cas No.:86583-19-9
Sample solution is provided at 25 µL, 10mM.
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3-Deazaadenosine (3-DZA) is an inhibitor of S-adenosylhomocysteine hydrolase (Ki = 3.9 μM).1,2 It has anti-inflammatory properties, inhibiting leukocyte adhesion and chemotaxis, lymphocyte-mediated cytolysis, phagocytosis, degranulation, and NF-κB signaling.3,4 3-DZA also has antiviral and antibacterial activities.1,5,6
1.Chiang, P.K.Biological effects of inhibitors of S-adenosylhomocysteine hydrolasePharmacol. Ther.77(2)115-134(1998) 2.Gordon, R.K., Ginalski, K., Rudnicki, W.R., et al.Anti-HIV-1 activity of 3-deaza-adenosine analogs: Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congenersEur. J. Biochem.270(17)3507-3517(2003) 3.Jurgensen, C.H., Huber, B.E., Zimmerman, T.P., et al.3-Deazaadenosine inhibits leukocyte adhesion and ICAM1 biosynthesis in tumor necrosis factor-stimulated human endothelial cellsJ. Immunol.144(2)653-661(1990) 4.Jeong, S.Y., Ahn, S.G., Lee, J.H., et al.3-Deazaadenosine, a S-adenosylhomocysteine hydrolase inhibitor, has dual effects on NF-κB regulationJ. Biol. Chem.274(27)18981-18988(1999) 5.Long, M.C., Allan, P.W., Luo, M.Z., et al.Evaluation of 3-deaza-adenosine analogues as ligands for adenosine kinase and inhibitors of Mycobacterium tuberculosis growthJ. Antimicrob. Chemother.59(1)118-121(2007) 6.Huggins, J., Zhang, Z.X., and Bray, M.Antiretroviral drug therapy of filovirus infections: S-adenosylhomocysteine hydrolase inhibitors inhibit ebola virus in vitro and in a lethal mouse modelJ. Infect. Dis.179(Suppl. 1)S240-S247(1999)
Cell experiment: | The HIV-1 strains A012 and A018 are used in the assay. Inhibition of p24 antigen is measured. Briefly, PHA-stimulated peripheral blood mononuclear (PBMCs) are incubated with either HIV-1 strain for 1 h at 37°C at 200-fold the 50% tissue culture infectious dose (TCID50) of the virus stock per 2 × 105 PBMC cells. The TCID50 is defined as the amount of virus stock at which 50% of the inoculated wells are positive. Cells are then grown in microtiter plates with different drug concentrations at 2 × 105 cells per well. On day 4, cells are resuspended and split 1:3 with fresh media and 3-Deazaadenosine. Supernatant p24 antigen is determined on day 7 by ELISA[1]. |
References: [1]. Gordon RK, et al. Anti-HIV-1 activity of 3-deaza-adenosine analogs. Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners. Eur J Biochem. 2003 Sep;270(17):3507-17. | |
| Cas No. | 86583-19-9 | SDF | |
| 别名 | c3Ado, deaza-Ado, 3-DZA | ||
| Canonical SMILES | O[C@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C3=C(C(N)=NC=C3)N=C2.Cl | ||
| 分子式 | C11H15ClN4O4 | 分子量 | 302.71 |
| 溶解度 | DMSO : 41.67 mg/mL (137.66 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3035 mL | 16.5175 mL | 33.0349 mL |
| 5 mM | 0.6607 mL | 3.3035 mL | 6.607 mL |
| 10 mM | 0.3303 mL | 1.6517 mL | 3.3035 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet