3-Methoxytyramine hydrochloride (3-O-methyl Dopamine hydrochloride)
(Synonyms: 2-甲氧基-4-(2-氨基乙基)苯酚,3-O-methyl Dopamine hydrochloride) 目录号 : GC33738
A natural metabolite of dopamine
Cas No.:1477-68-5
Sample solution is provided at 25 µL, 10mM.
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3-methoxy Tyramine is a natural metabolite of dopamine, produced by catechol O-methyltransferase (COMT). It is a weak agonist of trace amine-associated receptor 1 (EC50 = 1.7 ?M).1 3-methoxy Tyramine levels are assessed to monitor COMT activity in Parkinson’s disease.2 Elevated 3-methoxy Tyramine levels may also indicate abnormal catecholamine synthesis that occurs in certain neuroendocrine tumors.3
1.Wainscott, D.B., Little, S.P., Yin, T., et al.Pharmacologic characterization of the cloned human trace amine-associated receptor1 (TAAR1) and evidence for species differences with the rat TAAR1J. Pharmacol. Exp. Ther.320(1)475-485(2007) 2.Espinoza, S., Managó, F., Leo, D., et al.Role of catechol-O-methyltransferase (COMT)-dependent processes in Parkinson's disease and L-DOPA treatmentCNS Neurol. Disord. Drug Targets11(3)251-263(2012) 3.van Berkel, A., Lenders, J.W., and Timmers, H.J.Diagnosis of endocrine disease: Biochemical diagnosis of phaeochromocytoma and paragangliomaEur. J. Endocrinol.170(3)R910-R119(2014)
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Cell experiment: |
The rats are killed by cervical dislocation and the brains are removed and placed on an ice-cooled glass plate. The corpora striata are dissected out and homogenized immediately or stored at -17°C until analyzed. For the estimation of 3-Methoxytyramine hydrochloride 1 mL of the neutralized perchloric acid solution is placed in a glass test tube. One millilitre of a solution of potassium ferricyanide (20 μg/mL) in concentrated ammonium hydroxide is added. After 2 min 0 to 1 mL of a solution of cysteine (1 mg/mL) is added and mixed. The fluorescence of the resulting solution is measured in a spectrophotofluorometer fitted with an interference filter in the fluorescence light path. A blank determination is made by reversing the order of the addition of the ferricyanide-ammonia solution and the solution of cysteine. Authentic 3-Methoxytyramine hydrochloride is also added to a portion of the neutralized eluate and the fluorescence developed to check for quenching[1]. |
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Animal experiment: |
To assess effects of 3-Methoxytyramine hydrochloride (3-MT) in normal and TAAR1-KO mice, the animals are placed in the locomotor activity chamber and 30 min later various doses of 3-Methoxytyramine hydrochloride are administered i.c.v.. To perform i.c.v. administration in this paradigm, habituated mice are removed from the experimental chamber, briefly restrained, i.c.v. injection cannula is placed into the previously implanted (one week before) guide cannula and infusion of 3-Methoxytyramine hydrochloride or vehicle is performed for 4 minutes when animal is freely moving in a home cage. After infusion, animals are put back into experimental chamber and behavior is monitored for 90 min after administration[1]. |
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References: [1]. Sotnikova TD, et al. The dopamine metabolite 3-methoxytyramine is a neuromodulator. PLoS One. 2010 Oct 18;5(10):e13452. |
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| Cas No. | 1477-68-5 | SDF | |
| 别名 | 2-甲氧基-4-(2-氨基乙基)苯酚,3-O-methyl Dopamine hydrochloride | ||
| Canonical SMILES | COC1=C(O)C=CC(CCN)=C1.Cl | ||
| 分子式 | C9H14ClNO2 | 分子量 | 203.67 |
| 溶解度 | DMSO : 100 mg/mL (490.99 mM) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.9099 mL | 24.5495 mL | 49.099 mL |
| 5 mM | 0.982 mL | 4.9099 mL | 9.8198 mL |
| 10 mM | 0.491 mL | 2.455 mL | 4.9099 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet