4-Aminobenzylphosphonic Acid
(Synonyms: 4-氨基苄基膦酸) 目录号 : GC49494
An alkaline phosphatase inhibitor
Cas No.:5424-27-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
4-Aminobenzylphosphonic acid is an inhibitor of alkaline phosphatase (Ki = 1.1 mM for the bovine intestine enzyme).1 It has been used in affinity chromatography for the purification of alkaline phosphatase. 4-Aminobenzylphosphonic acid has also been used in electrochemical modification of glassy carbon electrodes.2
1.Landt, M., Boltz, S.C., and Butler, L.G.Alkaline phosphatase: Affinity chromatography and inhibition by phosphonic acidsBiochemistry17(5)915-919(1978) 2.Benjamin, O.D., Weissmann, M., and BÉlanger, D.Electrochemical modification of carbon electrode with benzylphosphonic groupsElectrochim. Acta122210-217(2014)
| Cas No. | 5424-27-1 | SDF | Download SDF |
| 别名 | 4-氨基苄基膦酸 | ||
| Canonical SMILES | O=P(O)(CC1=CC=C(C=C1)N)O | ||
| 分子式 | C7H10NO3P | 分子量 | 187.1 |
| 溶解度 | 1 M NH4OH: 1 mg/ml,DMF: Slightly soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.3447 mL | 26.7237 mL | 53.4474 mL |
| 5 mM | 1.0689 mL | 5.3447 mL | 10.6895 mL |
| 10 mM | 0.5345 mL | 2.6724 mL | 5.3447 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Rabbit antibodies with specificity for tyrosine phosphate are not reactive with tyrosine sulphate
Biosci Rep 1985 Aug;5(8):707-14.PMID:2415179DOI:10.1007/BF01117004
Tyrosine phosphate is a frequently observed modification in a limited number of protein molecules, principally the products of viral oncogenes and membrane receptors for mitogenic growth factors. Such a group is therefore an attractive candidate for affinity selection of such phosphoproteins by antibodies with specificity for tyrosine phosphate. This report demonstrates that antisera raised against 4-Aminobenzylphosphonic Acid have specificity for tyrosine phosphate, but are not inhibited by tyrosine sulphate. This observation has important ramifications for the detection and isolation of phosphotyrosine-containing proteins without copurification or detection of tyrosine sulphate-containing proteins, which are present in cells of all lineages. Furthermore, we demonstrate that antisera raised against the sulphanilate moiety are not reactive with tyrosine sulphate.