Δ4-Dafachronic Acid
(Synonyms: 3-keto-4-Cholestenoic Acid) 目录号 : GC17515A DAF-12 ligand that controls C. elegans development and lifespan
Cas No.:23017-97-2
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Δ4-Dafachronic acid is a DAF-12 activator.
Steroid hormone dafachronic acid (DA) has been identified to regulate dauer formation and lifespan in Caenorhabditis elegans via binding to the nuclear receptor DAF-12. DAF-12, an orphan nuclear hormone receptor, regulates dauer larva diapause, reproductive development, fat metabolism, as well as life cycle/longevity in C. elegans. In addition, DAF-12 is needed for entry into dauer by acting as a transcriptional repressor of target genes that favor reproductive development when it is not bound to ligand.
In vitro: Δ4-Dafachronic acid is a racemic version of a sterol-derived hormone acting as a ligand of DAF-12. In a previous study, it was found that there was no significant increase in the Δ4- and Δ4-dafachronic acid concentration in the long-lived glp-1 mutant, nor in the mutants defective in dauer formation [1]. Another previous study provided insights into the molecular interface between DA and IIS pathways and suggested that Δ4- and Δ7-dafachronic acid pathways had unique as well as overlapping biological functions in the control of development and lifespan [2].
In vivo: Up to now, there is no animal in vivo study reported for Δ4-Dafachronic acid.
Clinical trial: So far, no clinical study has been conducted.
References:
[1] Li TM et al. No Significant Increase in the Δ4- and Δ7-Dafachronic Acid Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation. G3 (Bethesda). 2015 May 12;5(7):1473-9.
[2] Dumas, K. J.,Guo, C.,Wang, X., et al. Functional divergence of dafachronic acid pathways in the control of C. elegans development and lifespan. Dev.Biol. 340(2), 605-612 (2010).
Cas No. | 23017-97-2 | SDF | |
别名 | 3-keto-4-Cholestenoic Acid | ||
化学名 | 3-oxo-cholest-4-en-26-oic acid | ||
Canonical SMILES | O=C1CC[C@@]2(C)C(CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@@H]4[C@@H](CCCC(C)C(O)=O)C)=C1 | ||
分子式 | C27H42O3 | 分子量 | 414.6 |
溶解度 | ≤12.5mg/ml in ethanol;2.5mg/ml in DMSO;5mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
||
Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.412 mL | 12.0598 mL | 24.1196 mL |
5 mM | 0.4824 mL | 2.412 mL | 4.8239 mL |
10 mM | 0.2412 mL | 1.206 mL | 2.412 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。