4-Hydroxymephenytoin
(Synonyms: 羟基香豆素) 目录号 : GC33522
A metabolite of mephenytoin
Cas No.:61837-65-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
(±)4-Hydroxymephenytoin is a metabolite of (S)-mephenytoin , a substrate of the cytochrome P450 (CYP) isoform CYP2C19 with anticonvulsant activities.1,2 It has been used for developing ultra-
1.Shimada, T., Misono, K.S., and Guengerich, F.P.Human liver microsomal cytochrome P-450 mephenytoin 4-hydroxylase, a prototype of genetic polymorphism in oxidative drug metabolismJ. Biol. Chem.261(2)909-921(1986) 2.Wong, P.T.H., Tan, S.F., and Lee, H.S.N-demethylation of methyl and dimethyl derivatives of phenytoin and their anticonvulsant activities in miceJpn. J. Pharmacol.48(4)473-478(1988) 3.Stewart, N.A., Buch, S.C., Conrads, T.P., et al.A UPLC-MS/MS assay of the "Pittsburgh cocktail": Six CYP probe-drug/metabolites from human plasma and urine using stable isotope dilutionAnalyst136(3)605-612(2011)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.2688 mL | 21.3438 mL | 42.6876 mL |
| 5 mM | 0.8538 mL | 4.2688 mL | 8.5375 mL |
| 10 mM | 0.4269 mL | 2.1344 mL | 4.2688 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。