4-Methylumbelliferyl-β-D-Glucopyranoside
(Synonyms: 4-甲基伞形酮酰-Β-D-吡喃葡糖酸苷,4-MU-GLU;4-MU-β-Gluc;4-MUG;MU-GLU) 目录号 : GC11870
A fluorescent substrate of β-glucosidase and β-glucocerebrosidase
Cas No.:18997-57-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease.1,2,3 Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4
References:
1. Hernández-Guzmán, A., Flores-Martínez, A., Ponce-Noyola, P., et al. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii FEBS Open Bio. 6(11), 1067-1077 (2016).
2. Kwapiszewski, R., Skolimowski, M., Ziółkowska, K., et al. A microfluidic device with fluorimetric detection for intracellular components analysis Biomed Microdevices 13(3), 431-440 (2011).
3. Shanmuganathan, M.K., and Britz-McKibbin, P. Inhibitor screening of pharmacological chaperones for lysosomal β-glucocerebrosidase by capillary electrophoresis Anal. Bioanal. Chem. 399(8), 2843-2853 (2011).
4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule J. Spectrosc. 1(1), 147128 (2013).
| Cas No. | 18997-57-4 | SDF | |
| 别名 | 4-甲基伞形酮酰-Β-D-吡喃葡糖酸苷,4-MU-GLU;4-MU-β-Gluc;4-MUG;MU-GLU | ||
| 化学名 | 4-methyl-7-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-2H-chromen-2-one | ||
| Canonical SMILES | OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O1 | ||
| 分子式 | C16H18O8 | 分子量 | 338.3 |
| 溶解度 | ≥ 84.5mg/mL in DMSO with gentle warming | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.956 mL | 14.7798 mL | 29.5596 mL |
| 5 mM | 0.5912 mL | 2.956 mL | 5.9119 mL |
| 10 mM | 0.2956 mL | 1.478 mL | 2.956 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。