4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG)
(Synonyms: 4-甲基伞形酮-Β-D-葡糖苷酸二水合物) 目录号 : GC30519
4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG) 是一种荧光底物 (Λex=362 nm, Λem=445 nm)。
Cas No.:881005-91-0
Sample solution is provided at 25 µL, 10mM.
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λex=362 nm , λem=445 nm).
4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat with λex=362 nm and λem=445 nm. The half-saturation constants, Km, that are calculated for sewage samples are close to the Km values calculated based on experimental plots presented previously for freshwaters[1].
[1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.
Kinase experiment: | GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement[1]. |
References: [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5. | |
| Cas No. | 881005-91-0 | SDF | |
| 别名 | 4-甲基伞形酮-Β-D-葡糖苷酸二水合物 | ||
| Canonical SMILES | O[C@H]([C@H]([C@@H]([C@@H](C(O)=O)O1)O)O)[C@@H]1OC2=CC=C(C(O3)=C2)C(C)=CC3=O.O | ||
| 分子式 | C16H18O10 | 分子量 | 370.31 |
| 溶解度 | DMSO : 150 mg/mL (405.07 mM);Water : 5.2 mg/mL (14.04 mM) | 储存条件 | Store at 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7004 mL | 13.5022 mL | 27.0044 mL |
| 5 mM | 0.5401 mL | 2.7004 mL | 5.4009 mL |
| 10 mM | 0.27 mL | 1.3502 mL | 2.7004 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet