5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein (hydrochloride)
(Synonyms: 5-([4,6-二氯三唑-2-基]胺)荧光素盐酸盐) 目录号 : GC49791
An amine-reactive fluorescent protein-labeling agent
Cas No.:21811-74-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein (DTAF) is an amine-reactive fluorescent probe for labeling proteins.1 It displays excitation/emission maxima of 492/513 nm, respectively. DTAF has been conjugated to IgG for use as fluorescent antibodies and as a pre-column or on-column reagent to label amino acids and amino acid-containing compounds.1,2,3 It has also been used as a derivatizing agent in the analysis of ephedrine and pseudoephedrine from various sources.3
1.Bakeslee, D., and Baines, M.G.Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgGJ. Immunol. Methods13(3-4)305-320(1976) 2.De Boer, S.H., and Wieczorek, A.Production of monoclonal antibodies to Corynebacterium spedonicumPhytopathology74(12)1431-1434(1984) 3.Wang, W., Li, C., Li, Y., et al.Rapid and ultrasensitive determination of ephedrine and pseudoephedrine derivatizated with 5-(4,6-dichloro-s-triazin-2-ylamino) fluorescein by micellar electrokinetic chromatography with laser-induced fluorescence detectionJ. Chromatogr. A.1102(1-2)273-279(2005)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8808 mL | 9.4038 mL | 18.8076 mL |
| 5 mM | 0.3762 mL | 1.8808 mL | 3.7615 mL |
| 10 mM | 0.1881 mL | 0.9404 mL | 1.8808 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。