5,6-Dihydrouridine
(Synonyms: 5,6-二氢尿苷) 目录号 : GC33502
A pyrimidine nucleoside and derivative of uridine
Cas No.:5627-05-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
5,6-Dihydrouridine is a pyrimidine nucleoside and derivative of uridine . It is present in, and provides conformational flexibility to, tRNA from bacteria, eukaryotes, and some archaea.1,2 5,6-Dihydrouridine inhibits E. coli cytidine deaminase in a cell-free assay (Ki = 3.4 ?M).3 Serum levels of 5,6-dihydrouridine are increased in patients with prostate cancer and positively correlated with lethality.4
1.Kasprzak, J.M., Czerwoniec, A., and Bujnicki, J.M.Molecular evolution of dihydrouridine synthasesBMC Bioinformatics13153(2012) 2.Dalluge, J.J., Hashizume, T., Sopchik, A.E., et al.Conformational flexibility in RNA: The role of dihydrouridineNucleic Acids Res.24(6)1073-1079(1996) 3.Cohen, R.M., and Woldender, R.Cytidine deaminase from Escherichia coli. Purification, properties and inhibition by the potential transition state analog 3,4,5,6-tetrahydrouridineJ. Biol. Chem.246(24)7561-7656(1971) 4.Huang, J., Mondul, A.M., Weinstein, S.J., et al.Prospective serum metabolomic profiling of lethal prostate cancerInt. J. Cancer145(12)3231-3243(2019)
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.0451 mL | 20.2257 mL | 40.4514 mL |
| 5 mM | 0.809 mL | 4.0451 mL | 8.0903 mL |
| 10 mM | 0.4045 mL | 2.0226 mL | 4.0451 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。