5(6)-FITC
(Synonyms: 异硫氰酸荧光素,Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers) 目录号 : GC33440
An amine-reactive fluorescent probe
Cas No.:27072-45-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fluorescein 5(6)-isothiocyanate (FITC) is an amine-reactive fluorescent probe.1,2 It reacts with amine groups to form conjugates with proteins for detection by various fluorescent-based applications. FITC displays excitation/emission maxima of 485/535 nm, respectively.2
1.Twining, S.S.Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymesAnal. Biochem.143(1)30-34(1984) 2.Chaganti, L.K., Venkatakrishnan, N., and Bose, K.An efficient method for FITC labelling of proteins using tandem affinity purificationBiosci. Rep.38(6)BSR20181764(2018)
| Cas No. | 27072-45-3 | SDF | |
| 别名 | 异硫氰酸荧光素,Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers | ||
| Canonical SMILES | OC1=CC=C(C2(O3)C(C=C(N=C=S)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(N=C=S)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6 | ||
| 分子式 | C21H11NO5S | 分子量 | 389.38 |
| 溶解度 | DMSO : ≥ 156 mg/mL (400.64 mM) | 储存条件 | Store at 2-8°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5682 mL | 12.8409 mL | 25.6819 mL |
| 5 mM | 0.5136 mL | 2.5682 mL | 5.1364 mL |
| 10 mM | 0.2568 mL | 1.2841 mL | 2.5682 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
The effects of fluorescent labels on Aβ42 aggregation detected by fluorescence correlation spectroscopy
Biopolymers 2018 Nov;109(11):e23237.PMID:30311215DOI:10.1002/bip.23237
Fluorescence-based methods are promising for measuring amyloid beta (Aβ) oligomers, given their capacity to analyse a sample at the single-molecule level. As the attachment of fluorescent labels may influence the biochemical properties of the Aβ oligomers, the effects of fluorescent labels on Aβ oligomers must be evaluated. In this paper, we compared the impacts of five different fluorescent dyes on the aggregation of Aβ42 oligomers using fluorescence correlation spectroscopy (FCS). We found that fluorescent labels of BODIPY® FL-C5 (BP), N-hydroxysuccinimide rhodamine B ester (RB) and rhodamine B isothiocyanate (RITC) increased the propensity of labelled Aβ42 oligomers to aggregate, whereas 6-(fluorescein-5-carboxamido) hexanoic acid succinimidyl ester (5-SFX) and fluorescein 5(6)-isothiocyanate (5(6)-FITC) decreased the propensity of labelled Aβ42 oligomers to aggregate. This difference originated from the different electric charges and hydrophobicity of the fluorescent dyes. These results provide valuable information for establishing different aggregation models for Aβ42 oligomers in vitro using FCS.