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5α,6β-Dihydroxycholestanol-d7 Sale

(Synonyms: Cholestanetriol-d7, 5α,6β-di-OHC-d7) 目录号 : GC46715

An internal standard for the quantification of 5α,6β-dihydroxycholestanol

5α,6β-Dihydroxycholestanol-d7 Chemical Structure

Cas No.:127684-07-5

规格 价格 库存 购买数量
500 μg
¥2,723.00
现货
1 mg
¥5,174.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

5α,6β-Dihydroxycholestanol-d7 is intended for use as an internal standard for the quantification of 5α,6β-dihydroxycholestanol by GC- or LC-MS. 5α,6β-Dihydroxycholestanol is an oxysterol metabolite of cholesterol formed from conversion of cholesterol epoxides by 5,6-epoxysterol hydrolase.1,2 It inhibits NMDA-mediated calcium influx in HEK293 cells expressing NR1/NR2B NMDA receptors in a concentration-dependent manner. It also binds to voltage-gated sodium (Nav) channels and decreases action potentials in hippocampal neurons in vitro when used at a concentration of 10 µM.2 It increases survival of spinal cord motoneurons, cortical neurons, and cerebellar granule neurons in vitro when used at concentrations ranging from 5 to 15 µM.3 5α,6β-Dihydroxycholestanol is neuroprotective in a rat model of cerebral ischemia when administered at a dose of 12 mg/kg and increases latency to seizure onset and reduces severity of seizures induced by pentylenetetrazole in rats. 5α,6β-Dihydroxycholestanol has been used as a replacement for cholesterol in the study of cholesterol binding proteins.4

1.Aringer, L., and Eneroth, P.Formation and metabolism in vitro of 5,6-epoxides of cholesterol and β-sitosterolJ. Lipid Res.15(4)389-398(1974) 2.Tang, L., Yan, M., Leng, T., et al.Cholestane-3β, 5α, 6β-triol suppresses neuronal hyperexcitability via binding to voltage-gated sodium channelsBiochem. Biophys. Res. Commun.496(1)95-100(2018) 3.Hu, H., Zhou, Y., Leng, T., et al.The major cholesterol metabolite cholestane-3β,5α,6β-triol functions as an endogenous neuroprotectantJ. Neurosci.34(34)11426-11438(2014) 4.Sheng, R., Kim, H., Lee, H., et al.Cholesterol selectively activates canonical Wnt signalling over non-canonical Wnt signallingNat. Commun.5:4393(2014)

Chemical Properties

Cas No. 127684-07-5 SDF
别名 Cholestanetriol-d7, 5α,6β-di-OHC-d7
Canonical SMILES C[C@H](CCCC(C([2H])([2H])[2H])([2H])C([2H])([2H])[2H])[C@@]1([H])CC[C@@]2([H])[C@]3([H])C[C@@H](O)[C@@]4(O)C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C
分子式 C27H41D7O3 分子量 427.7
溶解度 Methanol: Slightly Soluble 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 2.3381 mL 11.6904 mL 23.3809 mL
5 mM 0.4676 mL 2.3381 mL 4.6762 mL
10 mM 0.2338 mL 1.169 mL 2.3381 mL
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