5-(Hydroxymethyl)-2'-deoxycytidine
(Synonyms: 2'-脱氧-5-(羟甲基)胞啶; 5hmdC) 目录号 : GC40526A modified pyrimidine
Cas No.:7226-77-9
Sample solution is provided at 25 µL, 10mM.
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DNA methylation occurs mainly at the 5’-position of cytosine rings (5-methylcytosine) and occurs almost exclusively in CpG islands. Another epigenetic modification in DNA has been recently identified that involves hydroxymethylation of this same base (5-hydroxymethylcytosine), which potentially offers another level of transcriptional control.[1] 5-(Hydroxymethyl)-2’-deoxycytidine is a modified pyrimidine that is capable of producing interstrand cross-links in double-stranded DNA and has been used to quantify DNA hydroxymethylation levels in biological samples.[2],[3],[4]
Reference:
[1]. Song, C.X., Szulwach, K.E., Fu, Y., et al. Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine. Nat.Biotechnol. 29(1), 68-72 (2011).
[2]. Le, T., Kim, K.P., Fan, G., et al. A sensitive mass-spectrometry method for simultaneous quantification of DNA methylation and hydroxymethylation levels in biological samples. Analytical Biochemistry 412(2), 203-209 (2011).
[3]. Clark, T.A., Lu, X., Luong, K., et al. Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation. BMC Biol. 11(4), 1-10 (2013).
[4]. Krais, A.M., Park, Y.J., Plass, C., et al. Determination of genomic 5-hydroxymethyl-2'-deoxycytidine in human DNA by capillary electrophoresis with laser induced fluorescence. Epigenetics 6(5), 560-565 (2011).
| Cas No. | 7226-77-9 | SDF | |
| 别名 | 2'-脱氧-5-(羟甲基)胞啶; 5hmdC | ||
| 化学名 | 2'-deoxy-5-(hydroxymethyl)-cytidine | ||
| Canonical SMILES | O=C1N([C@H]2C[C@H](O)[C@@H](CO)O2)C=C(CO)C(N)=N1 | ||
| 分子式 | C10H15N3O5 | 分子量 | 257.2 |
| 溶解度 | 20mg/mL in DMSO, 5mg/mL in DMF | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.888 mL | 19.4401 mL | 38.8802 mL |
| 5 mM | 0.7776 mL | 3.888 mL | 7.776 mL |
| 10 mM | 0.3888 mL | 1.944 mL | 3.888 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet