5-Nitrobarbituric acid
目录号 : GC66324
5-Nitrobarbituric acid 是一种单纯疱疹病毒 1 型 (HSV-1) 抑制剂，IC50 为 1.7 μM。
Cas No.:480-68-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
5-Nitrobarbituric acid is a herpes simplex virus type-1 (HSV-1) inhibitor (IC50=1.7 μM)[1].
5-Nitrobarbituric acid can inhibit vero cell growth[1].
Cell Cytotoxicity Assay[1]
| Cell Line: | Vero cells |
| Concentration: | 0.06-5 μM |
| Incubation Time: | 2 hours |
| Result: | Showed high cytotoxicity to vero cells. |
| Cas No. | 480-68-2 | SDF | Download SDF |
| 分子式 | C4H3N3O5 | 分子量 | 173.08 |
| 溶解度 | DMSO : 100 mg/mL (577.77 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.7777 mL | 28.8884 mL | 57.7768 mL |
| 5 mM | 1.1555 mL | 5.7777 mL | 11.5554 mL |
| 10 mM | 0.5778 mL | 2.8888 mL | 5.7777 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Foundations of chemical microscopy. 1. Solid-state characterization of 5-Nitrobarbituric acid (dilituric acid) and its complexes with group IA and group IIA cations
J Pharm Biomed Anal 1997 May;15(8):1143-55.PMID:9215967DOI:10.1016/s0731-7085(96)01943-7.
5-Nitrobarbituric acid (dilituric acid) has been used as a chemical microscopic reagent for the qualitative identification of alkali metal (Group IA) and alkaline earth (Group IIA) cations. This methodology was based on the characterization of observed crystal morphologies, since a unique crystal habit could be associated with each adduct product. To understand the scientific foundations which permitted chemical microscopy to function as a useful analytical technique, the products formed between dilituric acid and the Group IA and IIA cations were characterized using polarizing optical microscopy, powder X-ray diffraction, thermal analysis and solid-state nuclear magnetic resonance. It was found that the origins of the different crystal morphologies associated with each of the adduct arose from the ability of the systems to form various hydrate species, which could also contain structural variations due to cation/diliturate packing patterns.
Foundations of chemical microscopy, 2 derivatives of primary phenylalkylamines with 5-Nitrobarbituric acid
J Pharm Biomed Anal 1999 May;19(6):865-75.PMID:10698552DOI:10.1016/s0731-7085(98)00142-3.
5-Nitrobarbituric acid (dilituric acid) was extensively used with great success as a chemical microscopic reagent for the qualitative identification of primary phenylalkylamines. This methodology was based on the characterization of observed crystal morphologies, since a unique crystal habit could be associated with each adduct product. To understand the scientific foundations which permitted chemical microscopy to function as a useful analytical technique, the products formed between dilituric acid and a series of primary phenylalkylamines were characterized using polarizing optical microscopy, powder X-ray diffraction, thermal analysis, and solid-state nuclear magnetic resonance. It was deduced that the origins of the different crystal morphologies associated with each of the crystalline adducts arose from the ability of the systems to form differing structural types and/or hydrates upon crystallization. The degree of hydration in the crystalline phenylalkylamine adducts appeared to increase as additional carbon atoms were added between the aromatic ring and the terminal amine group of the aliphatic sidechain.
Foundations of chemical microscopy. 3. Derivatives of some chiral phenylalkylamines and phenylalkylamino acids with 5-Nitrobarbituric acid
Chirality 2005 Feb;17(2):89-98.PMID:15641112DOI:10.1002/chir.20111.
At one time, 5-Nitrobarbituric acid (also known as dilituric acid) was extensively used as a chemical reagent for the qualitative identification of a variety of basic substances by light microscopy. This methodology was based on evaluation of observed crystal morphologies, because skilled observers could associate a unique crystal habit with the products formed with analytes when those were crystallized using standardized methods. As part of a study to understand the scientific foundations that permitted chemical microscopy to function as a useful analytical technique during its heyday, the products formed by dilituric acid with resolved and racemic phenylalkylamines and phenylalkylamino acids were characterized using a variety of physical analytical techniques. It was found that the different crystal morphologies associated with each of the crystalline adducts were derived from the ability of the systems to form differing structural types and/or hydrate crystal forms upon crystallization.
Structure-activity relationship of ligands of dihydrouracil dehydrogenase from mouse liver
Biochem Pharmacol 1989 May 1;38(9):1471-80.PMID:2719721DOI:10.1016/0006-2952(89)90187-1.
One hundred and five nucleobase analogues were screened as inhibitors of dihydrouracil dehydrogenase (DHUDase, EC 1.3.1.2) from mouse liver. 5-Benzyloxybenzyluracil, 1-deazauracil (2,6-pyridinediol), 3-deazauracil (2,4-pyridinediol), 5-benzyluracil, 5-Nitrobarbituric acid and 5,6-dioxyuracil (alloxan) were identified as potent inhibitors of this activity, with apparent Ki values of 0.2, 0.5, 2.1, 3.4, 3.8 and 6.6 microM respectively. Both 5-benzyloxybenzyluracil and 1-deazauracil were also potent inhibitors of DHUDase from human livers. These findings along with an extensive review of literature allowed the formulation of a structure-activity relationship. The binding to DHUDase required intact C2 and C4 oxo groups. Replacement of N1 or N3 by an endocyclic carbon enhanced binding. In contrast, replacement of C5 or C6 by an endocyclic nitrogen abolished binding. Addition of a charged group to C5 and/or C6, and of a hydrophobic group to C5 but not C6 improved the binding.
Drug uptake by brain. II. Barbiturate uptake alters the transport of amino acids in vitro(1)
Vopr Biokhim Mozga 1974;9:25-41.PMID:4471553doi
1. Valine and leucine uptake into mouse brain slices was stimulated by 1 to 4 mM phenobarbital and pentobarbital. Greater concentrations of these two barbiturates inhibited the transport of these amino acids. Barbital, isobarbituric acid, and 5-Nitrobarbituric acid had no effect, while amobarbital and secobarbital produced only inhibition of uptake. 2. Transport of glutamate and aspartate was unaffected by phenobarbital concentrations that inhibited uptake of lysine and alpha-aminoisobutyric acid. 3. Changes in the Na+ and K+ content of the slices were not related to stimulation of valine or leucine uptake. 4. There was no concentrative uptake of phenobarbital by brain slices. The partially saturable uptake of pentobarbital was not by active transport, because the uptake increased after heating brain, liver, or kidney slices to 95 degrees for 10 min. 5. This study shows that individual members of a pharmacologically related group may have specific effects on a composite physiological function (amino acid transport) of brain tissue.