5-Ph-IAA
目录号 : GC61525
5-phenyl-indole-3-acetic acid (5-Ph-IAA) 是 IAA 的衍生物。
Cas No.:168649-23-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
5-phenyl-indole-3-acetic acid (5-Ph-IAA) is a derivative of IAA. 5-Ph-IAA is a ligand to establish the auxin-induced degron 2 system (AID2) together with the OsTIR1 (F74G) mutant. Using AID2, degron fusion proteins can now be more precisely controlled, enabling target protein degradation with a half-life of 10 to 45 minutes by adding low doses of 5-Ph-IAA [1]. 5-Ph-IAA shows higher affinity to AtTIR1(F79G).
The mAID-fused targets were rapidly degraded by the addition of 1 μM 5-Ph-IAA and showed significant tumour suppression of mAID-BRD4 xenografts at all doses of 5-Ph-IAA treatment including 0, 1, 3, 10 mg/kg [1]. The treatment with 50 mM 5-Ph-IAA caused a rapid decrease in fluorescence intensity in many embryos (78%, 18/23 embryos), but 5 mM 5-Ph-IAA did not. 5-Ph-IAA can be used for loss-of-function experiments in C. elegans embryos, although treatment with 5-Ph-IAA is not always effective, possibly because the C. elegans embryo develops within the eggshell, which blocks its permeability to many compounds [2].
References:
[1].Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT. The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat Commun. 2020 Nov 11;11(1):5701.
[2].Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT. The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Genetics. 2022 Feb 4;220(2):iyab218.
5-phenyl-indole-3-acetic acid (5-Ph-IAA) 是 IAA 的衍生物。 5-Ph-IAA 是与 OsTIR1 (F74G) 突变体一起建立生长素诱导的 degron 2 系统 (AID2) 的配体。使用 AID2,现在可以更精确地控制 degron 融合蛋白,通过添加低剂量的 5-Ph-IAA [1] 使目标蛋白降解的半衰期为 10 至 45 分钟。 5-Ph-IAA对AtTIR1(F79G)具有更高的亲和力。
通过添加 1 μM 5-Ph-IAA,mAID 融合靶标会迅速降解,并且在所有剂量的 5-Ph-IAA 处理（包括 0、1、3、10毫克/千克 [1]。用 50 mM 5-Ph-IAA 处理导致许多胚胎（78%,18/23 胚胎）的荧光强度快速降低,但 5 mM 5-Ph-IAA 没有。 5-Ph-IAA 可用于秀丽隐杆线虫胚胎的功能丧失实验,尽管用 5-Ph-IAA 处理并不总是有效,这可能是因为秀丽隐杆线虫胚胎在蛋壳内发育,阻碍了其渗透性许多化合物 [2].
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.9796 mL | 19.8981 mL | 39.7962 mL |
| 5 mM | 0.7959 mL | 3.9796 mL | 7.9592 mL |
| 10 mM | 0.398 mL | 1.9898 mL | 3.9796 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。