6,8-Difluoro-7-hydroxy-4-methylcoumarin
(Synonyms: 6,8-二氟-7-羟基-4-甲基-2H-1-苯并吡喃-2-酮) 目录号 : GC49504
A fluorophore
Cas No.:215868-23-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
6,8-Difluoro-7-hydroxy-4-methylcoumarin is a fluorophore.1 It has been conjugated to streptavidin or secondary antibodies for flow cytometry and used to label bacteria for multi-color fluorescence in situ hybridization (FISH).2,1 6,8-Difluoro-7-hydroxy-4-methylcoumarin has also been coupled to peptides to improve electrospray ionization and MS sensitivity in MALDI-TOF MS.3 It displays absorption/emission maxima of 358/455 nm, respectively.4
1.Takada, T., Matsumoto, K., and Nomoto, K.Development of multi-color FISH method for analysis of seven Bifidobacterium species in human fecesJ. Microbiol. Methods58(3)413-421(2004) 2.Telford, W.G.Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodesCytometry A.61(1)9-17(2004) 3.Pashkova, A., Moskovets, E., and Karger, B.L.Coumarin tags for improved analysis of peptides by MALDI-TOF MS and MS/MS. 1. Enhancement in MALDI MS signal intensitiesAnal. Chem.76(15)4550-4557(2004) 4.Sun, W.C., Gee, K.R., and Haugland, R.P.Synthesis of novel fluorinated coumarins: Excellent UV-light excitable fluorescent dyesBioorg. Med. Chem. Lett.8(22)3107-3110(1998)
| Cas No. | 215868-23-8 | SDF | Download SDF |
| 别名 | 6,8-二氟-7-羟基-4-甲基-2H-1-苯并吡喃-2-酮 | ||
| Canonical SMILES | O=C1OC2=C(C(O)=C(C=C2C(C)=C1)F)F | ||
| 分子式 | C10H6F2O3 | 分子量 | 212.2 |
| 溶解度 | DMF: soluble,DMSO: soluble,Methanol: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.7125 mL | 23.5627 mL | 47.1254 mL |
| 5 mM | 0.9425 mL | 4.7125 mL | 9.4251 mL |
| 10 mM | 0.4713 mL | 2.3563 mL | 4.7125 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes
Bioorg Med Chem Lett 1998 Nov 17;8(22):3107-10.PMID:9873685DOI:10.1016/s0960-894x(98)00578-2
Two new fluorinated fluorescent dyes, 6,8-Difluoro-7-hydroxy-4-methylcoumarin (Marina Blue) and 3-carboxy-6,8-difluoro-7-hydroxycoumarin (Pacific Blue), exhibit excellent photophysical properties among a series of novel fluorinated 7-hydroxycoumarins. Most of these fluorinated coumarins have quantum yields (0.63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their lower pKaS and higher photostability, make them superior fluorescent dyes for use as reporter molecules in biological systems.