6''-O-Acetylsaikosaponin A
(Synonyms: 6''-O-乙酰基柴胡皂苷A) 目录号 : GC60534
6''-O-AcetylsaikosaponinA，从柴胡根中分离得到的乙酰柴胡皂苷，具有一定的破骨细胞抑制活性。
Cas No.:64340-46-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
6''-O-Acetylsaikosaponin A, an acetyl saikosaponin isolated from the roots of Bupleurum chinense, shows some osteoclast-inhibiting activities[1].
The major bioactive compounds isolated from R. bupleuri are saikosaponins, which have been proved to possess significant biological activities, including anti-hepatitis, anti-inflammatory, anti-tumor and immunoregulatory effects[2].
[1]. Yu JQ, et al. Osteoclast-inhibiting saikosaponin derivatives from Bupleurum chinense. Fitoterapia. 2013;85:101-108. [2]. Huang HQ, et al. Fast determination of saikosaponins in Bupleurum by rapid resolution liquid chromatography with evaporative light scattering detection. J Pharm Biomed Anal. 2009;49(4):1048-1055.
| Cas No. | 64340-46-1 | SDF | |
| 别名 | 6''-O-乙酰基柴胡皂苷A | ||
| Canonical SMILES | C[C@@]1(C[C@@H]2O)[C@]3(C=C[C@@]4([H])[C@]1(CC[C@]([C@@](C)5CO)([H])[C@@]4(CC[C@@H]5O[C@H](O[C@@H]6C)[C@@H]([C@H]([C@H]6O)O[C@@H]([C@@H]([C@H]7O)O)O[C@@H]([C@H]7O)COC(C)=O)O)C)C)[C@@](CC8(C)C)([H])[C@@]2(CC8)CO3 | ||
| 分子式 | C44H70O14 | 分子量 | 823.02 |
| 溶解度 | DMSO : 100 mg/mL (121.50 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.215 mL | 6.0752 mL | 12.1504 mL |
| 5 mM | 0.243 mL | 1.215 mL | 2.4301 mL |
| 10 mM | 0.1215 mL | 0.6075 mL | 1.215 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Fast determination of saikosaponins in Bupleurum by rapid resolution liquid chromatography with evaporative light scattering detection
J Pharm Biomed Anal 2009 May 1;49(4):1048-55.PMID:19201128DOI:10.1016/j.jpba.2009.01.011.
A rapid resolution liquid chromatography coupled with evaporative light scattering detection method was established for simultaneous determination of six saikosaponins, namely saikosaponin a, saikosaponin c, saikosaponin d, 6''-O-Acetylsaikosaponin A, 3''-O-acetylsaikosaponin d and 6''-O-acetylsaikosaponin d in Bupleurum. The analysis was performed by using an Agilent Zorbax SB-C18 column (1.8 microm, 3.0 mm x 50 mm i.d.) at gradient elution of water and acetonitrile, and the saikosaponins were well separated within 12 min, which provided about a fourfold reduction in analysis time by comparing a conventional high performance liquid chromatography method. Owing to their low ultraviolet absorption, the saikosaponins were detected by evaporative light scattering. The standard curves to quantify the saikosaponins were constructed by the log-log plot, which showed good linearity with the correlation coefficients exceeding 0.9954. The detection limits and quantification limits ranged in 8.38-25.00 microg/mL and 25.13-45.00 microg/mL, respectively. Satisfactory intra-day and inter-day precisions were achieved with the relative standard deviation (R.S.D.) less than 6.58%, and the average recoveries obtained were in the range of 96.9-100.4%. In addition, MeOH-1.0% (v/v) pyridine was found to be the best the extraction solvent when compared to MeOH and MeOH-1.0% (v/v) ammonia water. A total of 23 samples of roots of Bupleurum from different species or locations were examined with this analytical method, and their chemical profiles provided information for the chemotaxonomic investigation. The results demonstrated that the analytical method is highly effective for the quality evaluation of Bupleurum species.