6-Thioguanosine-5'-O-triphosphate (sodium salt)
(Synonyms: \r\n6-Thio-GTP) 目录号 : GC908176-巯基鸟嘌呤和氮芥的活性代谢物。
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
6-Thioguanosine-5’-O-triphosphate (6-thio-GTP) is an active metabolite of the purine analog 6-thioguanine and the immunosuppressive purine analog azathioprine .1,2 It is formed from 6-thioguanine via 6-thio-GMP and 6-thio-GDP intermediates by phosphorylation and from azathioprine via 6-mercaptopurine , 6-thio-GMP, and 6-thio-GDP intermediates by a non-enzymatic process and several enzymatic reactions. 6-thio-GTP binds to a variety of GTPases, including Rac, Ras, Cdc42, and RhoA but selectively inhibits activation of Rac1 and Rac2 over Cdc42 in primary human CD4+ T cells stimulated with anti-CD3 and anti-CD28 antibodies.3,4 It inhibits T cell receptor-stimulated proliferation of T cells and reduces CD28-dependent T cell survival in vitro in isolated mouse splenocytes.5 6-Thio-GTP (0.5 mg/kg) increases survival in a mouse model of heart transplantation.
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.5944 mL | 7.9719 mL | 15.9439 mL |
| 5 mM | 0.3189 mL | 1.5944 mL | 3.1888 mL |
| 10 mM | 0.1594 mL | 0.7972 mL | 1.5944 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。