7-hydroxy Methotrexate
(Synonyms: 7-羟基甲氨蝶呤) 目录号 : GC49051
A metabolite of methotrexate
Cas No.:5939-37-7
Sample solution is provided at 25 µL, 10mM.
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7-hydroxy Methotrexate (7-hydroxy MTX) is the major metabolite of the folic acid derivative MTX .1 It is formed by the 7-oxidation of MTX by hepatic aldehyde oxidases. Unlike MTX, 7-hydroxy MTX does not inhibit thymidylate synthase in isolated human acute myeloid and acute lymphoblastic leukemia cells.2 7-hydroxy MTX has a maximum tolerated dose of 0.1 g/kg, which is 30- to 50-fold lower than MTX, in rats.3 Serum levels of 7-hydroxy MTX have been used as a marker of MTX toxicity during chemotherapy.4,5
1.Johns, D.G., Iannotti, A.T., Sartorelli, A.C., et al.The relative toxicites of methotrexate and aminopterinBiochem. Pharmacol.15(5)555-561(1966) 2.Lingg, R.M., Hempel, G., Rots, M.G., et al.Effects and interaction of 7-hydroxy methotrexate and methotrexate in leukaemic cells ex vivo measured by the thymidylate synthase inhibition assayCancer Chemother. Pharmacol.56(3)322-327(2005) 3.FuskevÅg, O.M., Kristiansen, C., Lindal, S., et al.Maximum tolerated doses of methotrexate and 7-hydroxy-methotrexate in a model of acute toxicity in ratsCancer Chemother. Pharmacol.46(1)69-73(2000) 4.Schofield, R.C., Ramanathan, L.V., Murata, K., et al.Development of an assay for methotrexate and its metabolites 7-hydroxy methotrexate and DAMPA in serum by LC-MS/MSMethods in Molecular Biology213-222(2016) 5.Klapkova, E., Kukacka, J., Kotaska, K., et al.The influence of 7-OH methotrexate metabolite on clinical relevance of methotrexate determinationClin. Lab.57(7-8)599-606(2011)
| Cas No. | 5939-37-7 | SDF | |
| 别名 | 7-羟基甲氨蝶呤 | ||
| Canonical SMILES | NC1=C2C(NC(C(CN(C3=CC=C(C=C3)C(N[C@H](C(O)=O)CCC(O)=O)=O)C)=N2)=O)=NC(N)=N1 | ||
| 分子式 | C20H22N8O6 | 分子量 | 470.4 |
| 溶解度 | DMSO: slightly soluble,Ethanol: slightly soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1259 mL | 10.6293 mL | 21.2585 mL |
| 5 mM | 0.4252 mL | 2.1259 mL | 4.2517 mL |
| 10 mM | 0.2126 mL | 1.0629 mL | 2.1259 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet