7-Ketodeoxycholic acid
(Synonyms: 7-酮-3α,12-α-二羟基胆酸, 5β-Cholanic Acid-3α,12α-diol-7-one, 7-keto DCA) 目录号 : GC55814
7-Ketodeoxycholic acid是一种胆汁酸衍生物，是胆汁酸在梭菌（Clostridium absonum）中的代谢产物，也可由乳酸杆菌和双歧杆菌在特定条件下也可转化得到。
Cas No.:911-40-0
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
7-Ketodeoxycholic acid is a bile acid derivative that is a metabolite of bile acid in Clostridium absonum. It can also be converted by Lactobacillus and Bifidobacterium under certain conditions[1, 2]. Bile acids are natural steroid amphiphilic compounds formed by cholesterol oxidation in the liver and play a vital role in regulating lipid metabolism[3]. 7-Ketodeoxycholic acid levels are increased in the liver tissue of ethanol-induced alcoholic liver disease (ALD) mice[4]. Patients with non-alcoholic steatohepatitis (NASH) have higher levels of 7-Ketodeoxycholic acid in their plasma[5]. Resistant starch (LRS) can promote the excretion of 7-Ketodeoxycholic acid in hyperlipidemic rats[6].
References:
[1] Sutherland J D, Macdonald I A. The metabolism of primary, 7-oxo, and 7 beta-hydroxy bile acids by Clostridium absonum[J]. Journal of lipid research, 1982, 23(5): 726-732.
[2] Takahashi T, Morotomi M. Absence of cholic acid 7α-dehydroxylase activity in the strains of Lactobacillus and Bifidobacterium[J]. Journal of Dairy Science, 1994, 77(11): 3275-3286.
[3] Li T, Chiang J Y L. Regulation of bile acid and cholesterol metabolism by PPARs[J]. PPAR research, 2009, 2009(1): 501739.
[4] Charkoftaki G, Tan W Y, Berrios-Carcamo P, et al. Liver metabolomics identifies bile acid profile changes at early stages of alcoholic liver disease in mice[J]. 2022.
[5] Nimer N. Mass Spectrometry as Discovery Platform for Candidate Metabolite of Non-Alcoholic Steatohepatitis (NASH)[D]. Cleveland State University, 2020.
[6] Lei S, Liu L, Yue P, et al. Lotus seed resistant starch decreases the blood lipid and regulates the serum bile acids profiles in hyperlipidemic rats[J]. Journal of Functional Foods, 2022, 92: 105040.
7-Ketodeoxycholic acid是一种胆汁酸衍生物，是胆汁酸在梭菌（Clostridium absonum）中的代谢产物，也可由乳酸杆菌和双歧杆菌在特定条件下也可转化得到[1, 2]。胆汁酸是通过肝脏中胆固醇氧化形成的天然类固醇两亲化合物，在调节脂质代谢中发挥着至关重要的作用[3]。在乙醇诱导的酒精性肝病（ALD）小鼠的肝脏组织中，7-Ketodeoxycholic acid的水平升高[4]。非酒精性脂肪性肝炎（NASH）患者血浆中具有较高水平的7-Ketodeoxycholic acid[5]。在高脂血症大鼠中抗性淀粉（LRS）能够促进7-Ketodeoxycholic acid的排泄[6]。
| Cas No. | 911-40-0 | SDF | |
| 别名 | 7-酮-3α,12-α-二羟基胆酸, 5β-Cholanic Acid-3α,12α-diol-7-one, 7-keto DCA | ||
| 分子式 | C24H38O5 | 分子量 | 406.56 |
| 溶解度 | DMSO : 100 mg/mL (245.97 mM; Need ultrasonic) | 储存条件 | Store at -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4597 mL | 12.2983 mL | 24.5966 mL |
| 5 mM | 0.4919 mL | 2.4597 mL | 4.9193 mL |
| 10 mM | 0.246 mL | 1.2298 mL | 2.4597 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet