740 Y-P
(Synonyms: 740YPDGFR; PDGFR 740Y-P) 目录号 : GC16157
740 Y-P是一种PI3K激活剂，浓度为20μM 。
Cas No.:1236188-16-1
Sample solution is provided at 25 µL, 10mM.
-
Related Biological Data
Fu Fang Gang Liu liquid (FFGL) inhibits the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. D Cells were treated with FFGL (80 μg/mL) with various concentrations of 740 Y-P for 48 h. Cell viability was measured using the cell-counting kit 8 assay.
Similarly, we treated HeLa cells with the PI3K activator 740 Y-P (GlpBio, Montclair,CA, USA) (0, 12.5, 25, 50, 100, 200 μg/mL) in combination with FFGL (80 μg/mL) for 48 h.
Bmc Complement Med 23.1 (2023): 128. PMID: 37081536 IF: 2.838 -
Related Biological Data
THC inhibits the activation of the PI3K/AKT/mTOR pathway. g-i. Next, the same cells were treated for 48 hours to 15 μM 740Y-P (PI3K activator) alone, 100 μM THC alone, or 15 μM 740Y-P plus 100 μM THC.
We ordered the 740Y-P from GlpBio Technology (Montclair, CA, USA).
Arch Clin Psychiat 50.6 (2023). IF: 0.9839
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
|
Cell lines |
Human melanoma MNT-1 cells |
|
Preparation method |
Limited solubility. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
|
Reaction Conditions |
24 h |
|
Applications |
740 Y-P induces the generation of M6PR-positive puncta which is similar to the sucrose-induced vacuoles. 740 Y-P also prominently reduces the number of M6PR-positive vacuoles triggers by the sucrose treatment. |
|
References: 1. Bin BH, Bhin J, Yang SH et al. Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One. 2014 Aug 29;9(8):e105965. |
|
740 Y-P is an activator of PI3K with concentration of 20 μM [1].
PI3K (phosphoinositide 3-kinases, PI3Ks) is an enzyme and plays an important role in the fundamental cellular processes, such as vesicular trafficking, cell degranulation, cell migration, and glucose transporter. It has been reported that over-expression of PI3K was correlated with a variety kind of cancers [2].
740 Y-P is a potent PI3K activator and plays an important role in PI3K/AKT signaling pathway. When tested with human melanoma MNT-1 cells, 20 μM 740 Y-P for 24 hours treatment significantly reduced the number of M6PR-positive vacuoles induced by sucrose via activating PI3K [1]. In cerebellar granule cells in the circumstance of serum deprivation, 740 Y-P treatments reduced the cell death rate via binding to p85 which was correlated with PI 3-kinase-dependent phosphorylation of Akt process [3].
References:
[1]. Bin, B.H., et al., Hyperosmotic stress reduces melanin production by altering melanosome formation. PLoS One, 2014. 9(8): p. e105965.
[2]. Kong, B., et al., A subset of metastatic pancreatic ductal adenocarcinomas depends quantitatively on oncogenic Kras/Mek/Erk-induced hyperactive mTOR signalling. Gut, 2015.
[3]. Williams, E.J. and P. Doherty, Evidence for and against a pivotal role of PI 3-kinase in a neuronal cell survival pathway. Mol Cell Neurosci, 1999. 13(4): p. 272-80.
740 Y-P是一种PI3K激活剂，浓度为20μM [1]。PI3K（磷脂酰肌醇3-激酶）是一种酶，在细胞基本过程中发挥重要作用，如囊泡运输、细胞去颗粒化、细胞迁移和葡萄糖转运。已有报道称，PI3K的过表达与多种癌症相关[2]。 740 Y-P是一种有效的PI3K激活剂，在PI3K/AKT信号通路中发挥重要作用。当在人黑色素瘤MNT-1细胞中测试时，使用20μM 740 Y-P处理24小时可显着减少由蔗糖诱导的M6PR阳性液泡数量，并通过激活PI3K实现[1]。在血清匮乏情况下的小脑颗粒神经元中，使用740 Y-P处理可以通过结合与与 PI 3-kinase 相关联的 p85 来降低死亡率，并促进 Akt 进程上 PI 3-kinase依赖性的磷酸化[3]。
| Cas No. | 1236188-16-1 | SDF | |
| 别名 | 740YPDGFR; PDGFR 740Y-P | ||
| 分子式 | C141H222N43O39PS3 | 分子量 | 3270.72 |
| 溶解度 | ≥ 163.536mg/mL in DMSO | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.3057 mL | 1.5287 mL | 3.0574 mL |
| 5 mM | 0.0611 mL | 0.3057 mL | 0.6115 mL |
| 10 mM | 0.0306 mL | 0.1529 mL | 0.3057 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。