8-Bromo-cAMP, sodium salt
(Synonyms: 8-溴腺苷-3',5'-环单磷酸钠,8-Br-Camp sodium salt) 目录号 : GC16929
8-Bromo-cAMP (8-Bromo-cAMP, sodium salt)是一种细胞渗透性cAMP类似物，是cAMP依赖性蛋白激酶激活剂（PKA激活剂）,它能抑制肿瘤细胞生长，促进肿瘤分化。
Cas No.:76939-46-3
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
8-Bromo-cAMP (8-Bromo-cAMP, sodium salt) is a cell-permeable cAMP analogue that acts as a CAMP-dependent protein kinase activator (PKA activator). It has the ability to inhibit the growth of tumor cells and facilitate tumor differentiation [1-2].
8-Bromo-cAMP (20μM;24 h/48h) induced differentiation and apoptosis of human esophageal carcinoma cells Eca-109 [3]. 8-Bromo-cAMP (0.1/0.5 mM;10days) enhances the efficiency of inducing pluripotency in human fibroblast cells[4]. 8-Bromo-cAMP (250 μM) inhibits glucose transport activity in mouse placental cells in culture [5]. 8-Bromo-cAMP(0-1mM) induces a proliferative response in an IL-3 dependent leukemic cell line[6].
8-Bromo-cAMP (60 mg/kg/ day; 7 days; i.p) significantly inhibited the growth of CT26 tumor in mice[7]. 8-Bromo-cAMP enhances both humoral and cell-mediated immune responses induced by an HIV-1 DNA vaccine in mice[8].
References:
[1]. Chen TC, Hinton DR, et,al. Up-regulation of the cAMP/PKA pathway inhibits proliferation, induces differentiation, and leads to apoptosis in malignant gliomas. Lab Invest. 1998 Feb;78(2):165-74. PMID: 9484714.
[2]. Li H, Chen HC, et,al.Identification of a rapidly dephosphorylating 95-kDa protein as elongation factor 2 during 8-Br-cAMP treatment of N1E115 neuroblastoma cells. Biochem Biophys Res Commun. 1995 Dec 5;217(1):131-7. doi: 10.1006/bbrc.1995.2754. PMID: 8526900.
[3]. Wang HM, Zheng NG, et,al. Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109. World J Gastroenterol. 2005 Nov 7;11(41):6538-42. doi: 10.3748/wjg.v11.i41.6538. PMID: 16425431; PMCID: PMC4355801.
[4]. Wang Y, Adjaye J. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev Rep. 2011 Jun;7(2):331-41. doi: 10.1007/s12015-010-9209-3. PMID: 21120637.
[5]. Sakata M, Yamaguchi M, et,al. 8-Bromo-cAMP inhibits glucose transport activity in mouse placental cells in culture. J Endocrinol. 1996 Aug;150(2):319-27. doi: 10.1677/joe.0.1500319. PMID: 8869598.
[6]. Barge RM, Falkenburg JH, et,al. 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk 1,2 via a Shc-independent pathway. Biochim Biophys Acta. 1997 Feb 4;1355(2):141-6. doi: 10.1016/s0167-4889(96)00130-9. PMID: 9042334.
[7]. Wang S, Zhang Z, et,al. Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer. Onco Targets Ther. 2018 Jul 2;11:3765-3774. doi: 10.2147/OTT.S164982. PMID: 29997437; PMCID: PMC6033084.
[8]. Arai H, Xin KQ, et,al. 8 Br-cAMP enhances both humoral and cell-mediated immune responses induced by an HIV-1 DNA vaccine. Gene Ther. 2000 Apr;7(8):694-702. doi: 10.1038/sj.gt.3301145. PMID: 10800093.
8-Bromo-cAMP (8-Bromo-cAMP, sodium salt)是一种细胞渗透性cAMP类似物，是cAMP依赖性蛋白激酶激活剂（PKA激活剂）,它能抑制肿瘤细胞生长，促进肿瘤分化[1-2]。
8-Bromo-cAMP (20μM;24 h/48h)诱导人食管癌细胞Eca-109分化和凋亡[3]。8-Bromo-cAMP (0.1/0.5 mM;10days)可提高人成纤维细胞诱导多能性的效率[4]。8-Bromo-cAMP (250 μM)在培养小鼠胎盘细胞中抑制葡萄糖转运活性[5]。8-Bromo-cAMP (0-1mM)在IL-3依赖性白血病细胞系中诱导增殖反应[6]。
8-Bromo-cAMP (60 mg/kg/ day; 7 days; i.p)显著抑制小鼠CT26肿瘤的生长[7]。8-Bromo-cAMP可增强小鼠HIV-1 DNA疫苗诱导的体液和细胞介导的免疫反应[8]。
|
Cell experiment [1]: |
|
|
Cell lines |
Eca-109 cells (human esophageal carcinoma cells) |
|
Preparation method |
Cells were cultured with 8-Bromo-cAMP for 24 h and 48h. |
|
Reaction Conditions |
20μmol/L;24 h/48h |
|
Applications |
8-Bromo-cAMP treatment induced differentiation and apoptosis in Eca-109 cell. |
|
Animal experiment [2]: |
|
|
Animal models |
BALB/c mice |
|
Preparation method |
Mice underwent implantation of CT26 carcinoma tissue in their cecum. The experimental group received intraperitoneal injections of 8-Bromo-cAMP, while the control group was injected with normal saline. |
|
Dosage form |
60 mg/kg/ day; 7 days; i.p |
|
Applications |
8-Bromo-cAMP significantly inhibited the growth of CT26 tumor in mice. |
|
References: [1]. Wang HM, Zheng NG, et,al. Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109. World J Gastroenterol. 2005 Nov 7;11(41):6538-42. doi: 10.3748/wjg.v11.i41.6538. PMID: 16425431; PMCID: PMC4355801. [2]. Wang S, Zhang Z, et,al. Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer. Onco Targets Ther. 2018 Jul 2;11:3765-3774. doi: 10.2147/OTT.S164982. PMID: 29997437; PMCID: PMC6033084. |
|
| Cas No. | 76939-46-3 | SDF | |
| 别名 | 8-溴腺苷-3',5'-环单磷酸钠,8-Br-Camp sodium salt | ||
| 化学名 | sodium (4aR,6R,7R,7aS)-6-(6-amino-8-bromo-9H-purin-9-yl)-7-hydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide | ||
| Canonical SMILES | NC1=C(N=C(Br)N2[C@@H]3O[C@H](COP(O4)([O-])=O)[C@@H]4[C@H]3O)C2=NC=N1.[Na+] | ||
| 分子式 | C10H10BrN5NaO6P | 分子量 | 430.09 |
| 溶解度 | ≥ 43mg/mL in Water;DMSO : 250 mg/mL (581.29 mM; Need ultrasonic) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3251 mL | 11.6255 mL | 23.2509 mL |
| 5 mM | 0.465 mL | 2.3251 mL | 4.6502 mL |
| 10 mM | 0.2325 mL | 1.1625 mL | 2.3251 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet