8-hydroxy Mirtazapine
(Synonyms: 8-羟基米安色林) 目录号 : GC49544
A metabolite of mirtazapine
Cas No.:102335-57-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
8-hydroxy Mirtazapine is a metabolite of the antidepressant mirtazapine.1 It is formed from mirtazapine by the cytochrome P450 (CYP) isoform CYP2D6.
1.Dahl, M.-L., Voortman, G., Alm, C., et al.In vitro and in vivo studies on the disposition of mirtazapine in humansClin. Drug Investig.13(1)37-46(1997)
Cas No. | 102335-57-9 | SDF | Download SDF |
别名 | 8-羟基米安色林 | ||
Canonical SMILES | OC1=CN=C2C(CC3=CC=CC=C3C4N2CCN(C4)C)=C1 | ||
分子式 | C17H19N3O | 分子量 | 281.4 |
溶解度 | DMSO: slightly soluble,Methanol: slightly soluble w/ sonication | 储存条件 | -20°C |
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1 mg | 5 mg | 10 mg |
1 mM | 3.5537 mL | 17.7683 mL | 35.5366 mL |
5 mM | 0.7107 mL | 3.5537 mL | 7.1073 mL |
10 mM | 0.3554 mL | 1.7768 mL | 3.5537 mL |
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Enantioselective analysis of mirtazapine, demethylmirtazapine and 8-hydroxy Mirtazapine in human urine after solid-phase microextraction
J Sep Sci 2010 Feb;33(2):268-76.PMID:20087868DOI:10.1002/jssc.200900534.
A selective and reproducible off-line solid-phase microextraction procedure was developed for the simultaneous enantioselective determination of mirtazapine (MRT), demethylmirtazapine and 8-hydroxymirtazapine in human urine. CE was used for optimization of the extraction procedure whereas LC-MS was used for method validation and application. The influence of important factors in the solid-phase microextraction efficiency is discussed, such as the fiber coatings, extraction time, pH, ionic strength, temperature and desorption time. Before extraction, human urine samples were submitted to enzymatic hydrolysis at 37 degrees C for 16 h. Then, the enzyme was precipitated with trichloroacetic acid and the pH was adjusted to 8 with 1 mol/L pH 11 phosphate buffer solution. In the extraction, the analytes were transferred from the aqueous solution to the polydimethylsiloxane-divinylbenzene fiber coating and then desorbed in methanol. The mean recoveries were 5.4, 1.7 and 1.0% for MRT, demethylmirtazapine and 8-hydroxymirtazapine enantiomers, respectively. The method was linear over the concentration range of 62-1250 ng/mL. The within-day and between-day assay precision and accuracy were lower than 15%. The method was successfully employed in a preliminary cumulative urinary excretion study after administration of racemic MRT to a healthy volunteer.
Therapeutic drug monitoring of mirtazapine, desmethylmirtazapine, 8-hydroxymirtazapine, and mirtazapine-N-oxide by enantioselective HPLC with fluorescence detection
Ther Drug Monit 2006 Dec;28(6):760-5.PMID:17164691DOI:10.1097/FTD.0b013e31802c0264.
The tetracyclic antidepressant mirtazapine has been in clinical use for several years as a racemic drug. Because of a relatively narrow therapeutic index, therapeutic drug monitoring may be helpful to individually optimize therapy with mirtazapine. An enantioselective high-performance liquid chromatography (HPLC) method with fluorescence detection has been developed for the quantification of mirtazapine, desmethyl mirtazapine, 8-hydroxy Mirtazapine, and mirtazapine N-oxide. The method is suitable for the analysis of plasma and urine samples in the range from 1 to 100 ng/mL with precision (coefficient of variation, or CV) between 12% and 19%. The sample preparation step comprises a liquid-solid extraction procedure with good recoveries, between 85% and 99%. Patient samples for therapeutic drug monitoring as well as concentration-time series were assayed and the resulting enantiomer ratios analyzed. Typical trough levels were between 1 and 100 ng/mL, with enantiomer ratios of approximately 0.42 (S/R). In concentration-time series, enantiomer ratios distinctively greater than 1 were observed at early time points. Because the enantiomers of mirtazapine and desmethyl mirtazapine have different pharmacological properties, the method is believed to be helpful in understanding the concentration-effect relationships in the former.
Development of a novel ultrasonic-assisted magnetic dispersive solid-phase microextraction method coupled with high performance liquid chromatography for determination of mirtazapine and its metabolites in human urine and water samples employing experimental design
Anal Bioanal Chem 2016 Nov;408(27):7719-7729.PMID:27562753DOI:10.1007/s00216-016-9869-1.
Ultrasonic-assisted magnetic dispersive solid-phase microextraction coupled with high performance liquid chromatography has been developed for extraction and determination of mirtazapine, N-desmethyl mirtazapine, and 8-hydroxy Mirtazapine in human urine and water samples. Magnetic graphene oxide-polyaniline nanocomposite (MGOPA) as a novel SPME sorbent was synthesized and used for the microextraction process. The analytical performance of MGOPA was compared with magnetic graphene oxide nanocomposite and indicated that the new sorbent was quite effective for extraction of mirtazapine, N-desmethyl mirtazapine, and 8-hydroxy Mirtazapine. A two-stage experimental design approach, Plackett-Burman screening design and Box-Behnken optimization design, was used for screening and optimizing of significant variables in the microextraction process. The practical applicability of the proposed method was assessed by studying the linearity, intra-day and inter-day accuracy, enrichment factor, and precision. This method can be satisfactorily applied to the determination of mirtazapine and its metabolites in human urine and environmental water samples. Graphical Abstract Magnetic graphene oxide-polyaniline nanocomposite.