8-iso Prostaglandin A1
(Synonyms: 8-epi PGA1, 8-iso PGA1) 目录号 : GC41138An isoprostane
Cas No.:211186-29-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
8-iso Prostaglandin A1 (8-iso PGA1) is an isoprostane and a member in a large family of prostanoids of non-cyclooxygenase origin. It occurs as a common minor impurity in most commercial preparations of PGE1. The biological activity of 8-iso PGA1 has not been studied in depth or reported in the literature.
| Cas No. | 211186-29-7 | SDF | |
| 别名 | 8-epi PGA1, 8-iso PGA1 | ||
| Canonical SMILES | CCCCCC(O)/C=C/[C@H]1C=CC(=O)[C@H]1CCCCCCC(=O)O | ||
| 分子式 | C20H32O4 | 分子量 | 336.5 |
| 溶解度 | DMF: >75 mg/ml (from PGA1),DMSO: >50 mg/ml (from PGA1),Ethanol: >100 mg/ml (from PGA1),PBS pH 7.2: >2.4 mg/ml (from PGA1) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9718 mL | 14.8588 mL | 29.7177 mL |
| 5 mM | 0.5944 mL | 2.9718 mL | 5.9435 mL |
| 10 mM | 0.2972 mL | 1.4859 mL | 2.9718 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Crystal structure of Arabidopsis thaliana 12-oxophytodienoate reductase isoform 3 in complex with 8-iso prostaglandin A(1)
Proteins 2011 Nov;79(11):3236-41.PMID:21915915DOI:10.1002/prot.23153.
12-Oxophytodienoate reductase 3 (OPR3), one of the enzymes involved in the biosynthesis of the plant hormone jasmonic acid (JA), catalyzes the reduction of the cyclopentenone ring of (9S,13S)-12-oxophytodienoate [(9S,13S)-OPDA]. However, there has been no structural information about the interaction between OPRs and the physiologically relevant (9S,13S)-OPDA. Here we report the crystal structure of Arabidopsis thaliana OPR3 in complex with 8-iso Prostaglandin A1 (8-iso PGA1) which has the same stereochemistry in the cyclopentenone ring as in the physiologically relevant 9S,13S-OPDA. This structure reveals a new binding mode for substrate that likely contributes to the relaxed stereospecificity observed for AtOPR3.