8-Oxycoptisine
(Synonyms: 8-氧黄连碱) 目录号 : GC49275
An isoquinoline alkaloid with anticancer and gastroprotective activities
Cas No.:19716-61-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
8-Oxycoptisine is an isoquinoline alkaloid that has been found in Coptis chinensis and has anticancer and gastroprotective activities.1,2,3 It is an active metabolite of coptisine formed by gut microbiota.1 8-Oxycoptisine is cytotoxic against NCI-N87 gastric, but not Caco-2 colon, cancer cells (IC50s = 20.31 and >100 µM, respectively).2 It reverses resistance to paclitaxel in non-multidrug-resistant SKOV3 cells, as well as in HCT-15 cells, which endogenously express high levels of P-glycoprotein (P-gp), with EC50 values of 0.3 and 0.5 ng/ml, respectively.3 It reduces colonic shortening and inflammatory cell infiltration in a mouse model of ulcerative colitis induced by dextran sulfate when administered at doses of 50 or 100 mg/kg.1
1.Ai, G., Huang, Z., Cheng, J., et al.Gut microbiota-mediated transformation of coptisine Into a novel metabolite 8-oxocoptisine: Insight into its superior anti-colitis effectFront. Pharmacol.1212:639020(2021) 2.Qian, P., and Yang, X.-W.Five new alkaloids from Coptidis Rhizoma-Euodiae Fructus couple and their cytotoxic activities against gastrointestinal cancer cellsFitoterapia9374-80(2014) 3.Min, Y.D., Yang, M.C.M., Lee, K.H., et al.Protoberberine alkaloids and their reversal activity of P-gp expressed multidrug resistance (MDR) from the rhizome of Coptis japonica MakinoArch. Pharm. Res.29(9)757-761(2006)
| Cas No. | 19716-61-1 | SDF | |
| 别名 | 8-氧黄连碱 | ||
| Canonical SMILES | O=C1C2=C(C=C3C4=CC5=C(C=C4CCN13)OCO5)C=CC6=C2OCO6 | ||
| 分子式 | C19H13NO5 | 分子量 | 335.3 |
| 溶解度 | Chloroform: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.9824 mL | 14.912 mL | 29.824 mL |
| 5 mM | 0.5965 mL | 2.9824 mL | 5.9648 mL |
| 10 mM | 0.2982 mL | 1.4912 mL | 2.9824 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。