9(S)-HETE-d8
(Synonyms: 9(S)-Hydroxyeicosatetraenoic Acid-d8) 目录号 : GC46754
A neuropeptide with diverse biological activities
Cas No.:107656-13-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
9(S)-HETE-d8 is intended for use as an internal standard for the quantification of 9(S)-HETE by GC- or LC-MS. 9(S)-HETE is the (S) isomer of the monohydroxy fatty acid (±)9-HETE .1 It is formed from arachidonic acid by rat liver microsomal cytochrome P450 (CYP).2 9(S)-HETE is an agonist of retinoid X receptor γ (RXRγ), inducing RXRγ-dependent transcription when used at a concentration of 300 nM in BHK cell extracts expressing chick RXRγ.3
1.Schneider, C., Yu, Z., Boeglin, W.E., et al.Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatographyMethods Enzymol.433145-157(2007) 2.Capdevila, J., Yadagiri, P., Manna, S., et al.Absolute configuration of the hydroxyeicosatetraenoic acids (HETEs) formed during catalytic oxygenation of arachidonic acid by microsomal cytochrome P-450Biochem. Biophys. Res. Commun.141(3)1007-1011(1986) 3.Eager, N.S., Brickell, P.M., Snell, C., et al.Structural and functional evidence for activation of a chick retinoid X receptor by eicosanoidsProc. Biol. Sci.250(1327)63-69(1992)
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0441 mL | 15.2207 mL | 30.4414 mL |
| 5 mM | 0.6088 mL | 3.0441 mL | 6.0883 mL |
| 10 mM | 0.3044 mL | 1.5221 mL | 3.0441 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。