α-CGRP (human) (trifluoroacetate salt)
(Synonyms: α-Calcitonin Gene-Related Peptide (human), Calcitonin Gene Related Peptide-1 (human), CGRP-1 (human)) 目录号 : GC45199A neuropeptide with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
α-Calcitonin gene-related peptide (α-CGRP) is a neuropeptide with diverse biological activities. It belongs to the calcitonin family of peptides, which also includes amylin , calcitonin , and adrenomedullin. α-CGRP binds selectively to a heteromeric complex of calcitonin receptor-like receptor (CRLR) and receptor activity-modifying protein 1 (RAMP1; IC50 = 0.1 nM) over a complex of CLCR and RAMP2 (IC50 = 1,300 nM). It also binds selectively to complexes of human calcitonin receptor 2 (CTR2) and RAMP1 (IC50 = 43.7 nM) compared to CTR2 and RAMP2 complexes (IC50 = 141.3 nM) in amylin radioligand displacement assays. α-CGRP induces cAMP-activated currents in Xenopus oocytes with endogenous Cgrp receptors that overexpress RAMP1 (EC50 = 9 nM). It also induces cAMP accumulation in COS-7 cells transfected with both CTR2 and RAMP1 (EC50 = 8.32 nM).
Cas No. | SDF | ||
别名 | α-Calcitonin Gene-Related Peptide (human), Calcitonin Gene Related Peptide-1 (human), CGRP-1 (human) | ||
分子式 | C163H267N51O49S2•XCF3COOH | 分子量 | 3789.3 |
溶解度 | Water: 1 mg/ml | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.2639 mL | 1.3195 mL | 2.639 mL |
5 mM | 0.0528 mL | 0.2639 mL | 0.5278 mL |
10 mM | 0.0264 mL | 0.132 mL | 0.2639 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。