α-Zearalanol
(Synonyms: 折仑诺) 目录号 : GC40753
α-Zearalenol(α-玉米赤烯醇)是一种对雌激素受体 (ER) 具有高亲和力的霉菌毒素, α-Zearalenol 是 zearalenone (ZEN) 的衍生物,由于其异种雌激素效应可引起动物的生殖障碍。
Cas No.:26538-44-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
|
Cell lines |
T47D cell |
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Preparation Method |
α-Zearalenol are added to the medium. |
|
Reaction Conditions |
10-4M to 10-14 M;4days |
|
Applications |
α-Zearalenol at low concentrations (10-8-10-9 M) stimulated T47D cell growth. |
| Animal experiment [2]: | |
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Animal models |
Male rat |
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Preparation Method |
Male mice were injected α-Zearalenol intraperitoneally, then cage with sexually mature untreated female mice, tested for pregnancy the next day, and finally evaluated for semen quality and fertility. |
|
Dosage form |
0,25,50 and 75mg/kg once a day for 7 days; i.p. |
|
Applications |
The proportion of malformed sperm increased significantly in the A-Zol-treated male mice compared with the control group, while the proportion of live sperm decreased significantly and the acrosome integrity rate of sperm decreased. |
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References: [1].Khosrokhavar R, Rahimifard N, et,al. Effects of zearalenone and alpha-Zearalenol in comparison with Raloxifene on T47D cells. Toxicol Mech Methods. 2009 Mar;19(3):246-50. doi: 10.1080/15376510802455347. PMID: 19730705; PMCID: PMC2736542. | |
α-Zearalenol (α-zol) is a Mycotoxin with high affinity for the estrogen receptors (ER), α-Zearalenol is the derivative of zearalenone (ZEN), causes reproductive disorders in animals, due to its xenoestrogenic effects[1-3].
α-Zearalenol (0.001-10 µM; 24-72 hours) induces an increase of cell viability on MCF-7 cells mainly after 72 h of treatment whereas it is slightly cytotoxic on MDA-MB231 cells[4]. α-Zearalenol at low concentrations (10-8-10-9 M;4days) stimulated T47D cell growth[5]. α-Zearalenol(10-10, 10-8 and 10-6 M; 24, 48 and 72 h) was able to increase the protein biosynthesis as well as the lipid metabolism in MCF-7 cells, and, hence, to induce an estrogen positive breast cancer progression[6].
α-Zearalenol (0,25,50 and 75mg/kg once a day for 7 days; i.p.) increased the proportion of malformed sperm, decreased the proportion of live sperm, and decreased acrosome integrity of sperm. α-Zearalenol has a significant harmful effect on the reproductive system of male mice [7].
References:
[1]. Tatay E, EspÍn S,et,al. Estrogenic activity of zearalenone, α-zearalenol and β-zearalenol assessed using the E-screen assay in MCF-7 cells. Toxicol Mech Methods. 2018 May;28(4):239-242. doi: 10.1080/15376516.2017.1395501. Epub 2017 Nov 10. PMID: 29057713.
[2]. Ueno Y, Tashiro F. alpha-Zearalenol, a major hepatic metabolite in rats of zearalenone, an estrogenic mycotoxin of Fusarium species. J Biochem. 1981 Feb;89(2):563-71. doi: 10.1093/oxfordjournals.jbchem.a133232. PMID: 6453865.
[3]. Zielonka Ł, Gajęcka M, et,al.The Effect of Different Doses of Zearalenone in Feed on the Bioavailability of Zearalenone and Alpha-Zearalenol, and the Concentrations of Estradiol and Testosterone in the Peripheral Blood of Pre-Pubertal Gilts. Toxins (Basel). 2020 Feb 26;12(3):144. doi: 10.3390/toxins12030144. PMID: 32111008; PMCID: PMC7150765.
[4]. Marin DE, Pistol GC, et,al. Cytotoxic and inflammatory effects of individual and combined exposure of HepG2 cells to zearalenone and its metabolites. Naunyn Schmiedebergs Arch Pharmacol. 2019 Aug;392(8):937-947. doi: 10.1007/s00210-019-01644-z. Epub 2019 Mar 27. PMID: 30919009.
[5]. Khosrokhavar R, Rahimifard N, et,al.Effects of zearalenone and alpha-Zearalenol in comparison with Raloxifene on T47D cells. Toxicol Mech Methods. 2009 Mar;19(3):246-50. doi: 10.1080/15376510802455347. PMID: 19730705; PMCID: PMC2736542.
[6]. Nittoli AC, Costantini S, et,al.Effects of α-zearalenol on the metabolome of two breast cancer cell lines by 1H-NMR approach. Metabolomics. 2018 Feb 14;14(3):33. doi: 10.1007/s11306-018-1330-3. PMID: 30830360.
[7]. Yang Jianying, Cui Sheng.Toxicity of zearalenone and its metabolite α-Zearalenol on reproductive system of male mice [C]// Animal Physiology and Biochemistry Branch of Chinese Society of Animal Husbandry and Veterinary Medicine.A compilation of abstracts of the Ninth National Academic Exchange on Animal Physiology and Biochemistry.[Publisher unknown],2006:155.
α-Zearalenol(α-玉米赤烯醇)是一种对雌激素受体 (ER) 具有高亲和力的霉菌毒素, α-Zearalenol 是 zearalenone (ZEN) 的衍生物,由于其异种雌激素效应可引起动物的生殖障碍[1-3]。
α-玉米赤烯醇(0.001-10 µM;24-72h)主要在72小时后诱导MCF-7细胞活性的增加,而它对MDA-MB231细胞有轻微的细胞毒性[4]。低浓度α-玉米赤烯醇(10-8-10-9 M;4天)刺激T47D细胞生长[5]。α-玉米赤烯醇(10-10, 10-8 and 10-6 M; 24, 48 and 72 h)能够增加MCF-7细胞中的蛋白质生物合成和脂质代谢,从而诱导雌激素阳性乳腺癌进展[6]。
α-玉米赤烯醇(0,25,50 and 75mg/kg once a day for 7 days; i.p.)处理的雄性小鼠畸形精子比例明显增加,而活精子比例明显下降,精子顶体完整性率下降。α-玉米赤烯醇对雄性小鼠生殖系统有明显的危害作用[7]。
| Cas No. | 26538-44-3 | SDF | |
| 别名 | 折仑诺 | ||
| Canonical SMILES | OC1=C(C(O[C@@H](C)CCC[C@H](O)CCCCC2)=O)C2=CC(O)=C1 | ||
| 分子式 | C18H26O5 | 分子量 | 322.4 |
| 溶解度 | DMSO : 100 mg/mL (310.17 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1017 mL | 15.5087 mL | 31.0174 mL |
| 5 mM | 0.6203 mL | 3.1017 mL | 6.2035 mL |
| 10 mM | 0.3102 mL | 1.5509 mL | 3.1017 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。