β-Aminopropionitrile
(Synonyms: 3-氨基丙腈,BAPN) 目录号 : GC61983
β-Aminopropionitrile（BAPN）是一种特异的不可逆赖氨酸氧化酶（LOX）抑制剂，IC50值约为3-8μM。
Cas No.:151-18-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment [1]: |
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Cell lines |
HeLa and SiHa cells |
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Preparation method |
Both cell lines were incubated on Matrigel-coated filters of transwells for 48h under normoxia or hypoxia in the presence or absence of 500μM β-Aminopropionitrile. |
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Reaction Conditions |
500μM; 48 h |
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Applications |
In the presence of 500 μM β-Aminopropionitrile, the cancer cell invasion ability of HeLa and SiHa cells was inhibited to 50% and 60% of the control, respectively. |
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Animal experiment [2]: |
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Animal models |
Male Wistar rats |
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Preparation method |
Male Wistar rats were fed either a HFD or a standard diet for 6 weeks. Half of the animals of each group received β-Aminopropionitrile (100mg/kg/day) in the drinking water for the same period. The amount of β-Aminopropionitrile effectively taken daily per animal was calculated from the amount of water consumed on a daily basis. |
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Dosage form |
100 mg/kg; accepted in drinking water |
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Applications |
Treatment with β-Aminopropionitrile improved fasted glucose and insulin levels and consequently reduced homeostasis model assessment (HOMA) index in the fed a high-fat diet (HFD) group. |
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References: [1]Yang X, Li S, Li W, et al. Inactivation of lysyl oxidase by β-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells[J]. Oncology reports, 2013, 29(2): 541-548. [2]Miana M, Galán M, Martínez-Martínez E, et al. The lysyl oxidase inhibitor β-aminopropionitrile reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats[J]. Disease models & mechanisms, 2015, 8(6): 543-551. |
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β-Aminopropionitrile (BAPN) is a specific irreversible lysine oxidase (LOX) inhibitor with an IC50 value of approximately 3-8μM [1]. β-Aminopropionitrile targets the active site of LOX or LOXL isoenzymes [2]. β-Aminopropionitrile can cross-link elastin fibers and collagen fibers to maintain the homeostasis of the elastic layer [3].
In vitro, β-Aminopropionitrile (500μM) treated cervical cancer HeLa and SiHa cells for 48 hours, inactivating LOX activity and significantly inhibiting hypoxia-induced cell invasion. The invasion ability of the two cell lines was inhibited by 50% and 60% respectively[4]. β-Aminopropionitrile (1.0mg/mL, approximately 14.3μM) treated LX-2 cells for 24 hours and significantly down-regulated the expression of LOXL2 and α-SMA induced by TGF β [5].
In vivo, β-Aminopropionitrile (100 mg/kg) added to the drinking water of rats fed a high-fat diet for 6 weeks reduced fasting blood glucose and insulin levels, reduced homeostasis model assessment (HOMA) index, and improved obesity and adipose tissue fibrosis. phenomenon [6]. Adding β-Aminopropionitrile (1000 mg/kg) to drinking water and feeding C57BL/6 male mice for 4 weeks significantly reduced the weight of the mice and reduced plasma triglyceride and cholesterol levels [7].
References:
[1] Bhave G, Cummings C F, Vanacore R M, et al. Peroxidasin forms sulfilimine chemical bonds using hypohalous acids in tissue genesis[J]. Nature chemical biology, 2012, 8(9): 784-790.
[2] Rodriguez H M, Vaysberg M, Mikels A, et al. Modulation of lysyl oxidase-like 2 enzymatic activity by an allosteric antibody inhibitor[J]. Journal of biological chemistry, 2010, 285(27): 20964-20974.
[3] Kawai T, Takayanagi T, Forrester S J, et al. Vascular ADAM17 (a Disintegrin and Metalloproteinase Domain 17) Is Required for Angiotensin II/β-Aminopropionitrile–Induced Abdominal Aortic Aneurysm[J]. Hypertension, 2017, 70(5): 959-963.
[4] Yang X, Li S, Li W, et al. Inactivation of lysyl oxidase by β-aminopropionitrile inhibits hypoxia-induced invasion and migration of cervical cancer cells[J]. Oncology reports, 2013, 29(2): 541-548.
[5] Cheng F, Yang F, Wang Y, et al. Mesenchymal stem cell-derived exosomal miR-27b-3p alleviates liver fibrosis via downregulating YAP/LOXL2 pathway[J]. Journal of Nanobiotechnology, 2023, 21(1): 195.
[6] Miana M, Galán M, Martínez-Martínez E, et al. The lysyl oxidase inhibitor β-aminopropionitrile reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats[J]. Disease models & mechanisms, 2015, 8(6): 543-551.
[7] Ren W, Liu Y, Wang X, et al. β-Aminopropionitrile monofumarate induces thoracic aortic dissection in C57BL/6 mice[J]. Scientific reports, 2016, 6(1): 28149.
β-Aminopropionitrile（BAPN）是一种特异的不可逆赖氨酸氧化酶（LOX）抑制剂，IC50值约为3-8μM[1]。β-Aminopropionitrile 靶向LOX或LOXL同工酶的活性位点[2]。β-Aminopropionitrile可使弹性蛋白纤维和胶原纤维交联，维持弹性层的稳态[3]。
在体外，β-Aminopropionitrile（500μM）处理宫颈癌HeLa和SiHa细胞48h，灭活了LOX活性，显著抑制了缺氧诱导的细胞侵袭，两种细胞系的侵袭能力分别被抑制了50%和60%[4]。β-Aminopropionitrile（1.0 mg/mL，约14.3μM）处理 LX-2细胞24h，显著下调了TGF β诱导的LOXL2和 α-SMA表达[5]。
在体内，β-Aminopropionitrile（100mg/kg）加入到高脂饮食大鼠的饮用水中喂养6周，降低了大鼠空腹血糖和胰岛素水平，降低了稳态模型评估（HOMA）指数，并改善了肥胖和脂肪组织纤维化现象[6]。β-Aminopropionitrile（1000mg/kg）加入到饮用水中喂养C57BL/6 雄性小鼠4周，显著减轻了小鼠体重，降低了血浆甘油三酯和胆固醇水平[7]。
| Cas No. | 151-18-8 | SDF | |
| 别名 | 3-氨基丙腈,BAPN | ||
| Canonical SMILES | N#CCCN | ||
| 分子式 | C3H6N2 | 分子量 | 70.09 |
| 溶解度 | DMSO : 130 mg/mL (1854.76 mM)|Water : 50 mg/mL (713.37 mM) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 14.2674 mL | 71.3369 mL | 142.6737 mL |
| 5 mM | 2.8535 mL | 14.2674 mL | 28.5347 mL |
| 10 mM | 1.4267 mL | 7.1337 mL | 14.2674 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。