β-Asarone
(Synonyms: β-细辛脑) 目录号 : GC40574
β-Asarone是石菖蒲根茎的主要成分，对中枢神经系统有显著的药理作用。
Cas No.:5273-86-9
Sample solution is provided at 25 µL, 10mM.
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β-Asarone is a major component of Acorus tatarinowii Schott rhizomes, has significant pharmacological effects on the central nervous system (CNS) [1-3]. β-Asarone could pass the blood-brain barrier (BBB) [4]. β-asarone inhibits MAO-A (IC50 = 142 ± 18 µM), and MAO-B (IC50 = 362 ± 43 µM).
β-Asarone (30 µg/mL) potentiates the nerve growth factor (NGF)-induced neuronal differentiation through the phosphorylation of CREB by the activation of protein kinase-A pathway in PC12 cells [6]. β-Asarone had neuroprotective activity in LPS activated BV-2 microglia cells and depression [2,7-8].
β-Asarone (20 and 30 mg/kg/d) improved the neurological outcome after cerebral ischemia and reperfusion in terms of neurobehavioral function in rats [9]. β-Asarone improves cognitive function by suppressing neuronal apoptosis in the beta-amyloid hippocampus injection rats. [10].
References:
[1]. Cho J, Kim YH, Kong JY, Yang CH, Park CG. Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus. Life Sciences. 2002 Jun 21;71(5):591-9.
[2]. Chen YZ, Wang QW, Liang Y, Fang YQ. Protective effects of beta-asarone on cultured rat cortical neurons damage induced by glutamate. Zhong yao cai= Zhongyaocai= Journal of Chinese Medicinal Materials. 2007 Apr 1;30(4):436-9.
[3]. Fang YQ, Fang RM, Fang GL, Jiang Y, Fu SY. Effects of beta-asarone on expression of c-fos in kindling epilepsy rat brain. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica. 2008 Mar 1;33(5):534-6.
[4]. Fang YQ, Wei G. To analyze if the Rhizoma Acori Tatarimowii naph-tha can go through the BBB or not with GC-MS. Zhong Yao Xin Yao Yu: Ling Chuang Yao Li. 2002;3:181-2.
[5]. Tao G, Irie Y, Li DJ, Keung WM. Eugenol and its structural analogs inhibit monoamine oxidase A and exhibit antidepressant-like activity. Bioorganic & medicinal chemistry. 2005 Aug 1;13(15):4777-88.
[6]. Lam KY, Chen J, Lam CT, Wu Q, Yao P, Dong TT, Lin H, Tsim KW. Asarone from Acori Tatarinowii Rhizoma potentiates the nerve growth factor-induced neuronal differentiation in cultured PC12 cells: a signaling mediated by protein kinase A. PLoS One. 2016 Sep 29;11(9):e0163337.
[7]. Li C, Xing G, Dong M, Zhou L, Li J, Wang G, Zou D, Wang R, Liu J, Niu Y. Beta-asarone protection against beta-amyloid-induced neurotoxicity in PC12 cells via JNK signaling and modulation of Bcl-2 family proteins. European Journal of Pharmacology. 2010 Jun 10;635(1-3):96-102.
[8].Lim HW, Kumar H, Kim BW, More SV, Kim IW, Park JI, Park SY, Kim SK, Choi DK. β-Asarone (cis-2, 4, 5-trimethoxy-1-allyl phenyl), attenuates pro-inflammatory mediators by inhibiting NF-κB signaling and the JNK pathway in LPS activated BV-2 microglia cells. Food and chemical toxicology. 2014 Oct 1;72:265-72.
[9]. Yang YX, Chen YT, Zhou XJ, Hong CL, Li CY, Guo JY. Beta-asarone, a major component of Acorus tatarinowii Schott, attenuates focal cerebral ischemia induced by middle cerebral artery occlusion in rats. BMC Complementary and Alternative Medicine. 2013 Dec;13:1-6.
[10]. Geng Y, Li C, Liu J, Xing G, Zhou L, Dong M, Li X, Niu Y. Beta-asarone improves cognitive function by suppressing neuronal apoptosis in the beta-amyloid hippocampus injection rats. Biological and Pharmaceutical Bulletin. 2010 May 1;33(5):836-43.
| Cell experiment [1]: | |
|
Cell lines |
A spontaneously immortalized rat Schwann cell line RSC96 |
|
Preparation Method |
RSC96 cells treated with 0, 5, 10, and 20 µM β-asarone for 2, 4, and 6 d. Cells were digested by 0.25% trypsin and then resuspended in PBS containing 60 µg/mL proteinase K for 6 h at 60°C. |
|
Reaction Conditions |
0, 5, 10, and 20 µM for 2, 4, and 6 d |
|
Applications |
Cells cultured with β-asarone grew faster than those in the control group. Among the three concentrations, 10-µM β-asarone exhibited the strongest promoting effect on cell growth in the same time point of culture. |
| Animal experiment [2]: | |
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Animal models |
adult Wistar rats |
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Preparation Method |
The animals were separated into five groups of ten rats each. The first group served as sham (SHAM). The second group was the ischemic group (MCAO). Group I and group II were treated orally by distilled water for 30 days respectively. Group III (β-Asarone-10), Group IV (β-Asarone-20) and Group V (β-Asarone-30) were treated orally by β-Asarone (10, 20 and 30 mg/kg/day, respectively) for 30 days followed by middle cerebral artery occlusion (MCAO) induced cerebral ischemia. |
|
Dosage form |
10, 20 and 30 mg/kg/day, oral, for 30 days. |
|
Applications |
The β-Asarone-20 group and β-Asarone-30 group significantly suppressed the development of behavioral abnormality as compared with the MCAO group. |
|
References: [1]: Xu F, Wu H, Zhang K, Lv P, Zheng L, Zhao J. Pro-neurogenic effect of β-asarone on RSC96 Schwann cells in vitro. In Vitro Cellular & Developmental Biology-Animal. 2016 Mar;52:278-86. | |
| Cas No. | 5273-86-9 | SDF | |
| 别名 | β-细辛脑 | ||
| Canonical SMILES | COC1=CC(/C=C\C)=C(OC)C=C1OC | ||
| 分子式 | C12H16O3 | 分子量 | 208.3 |
| 溶解度 | DMF: 16 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 11 mg/ml,Ethanol: 14 mg/ml | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.8008 mL | 24.0038 mL | 48.0077 mL |
| 5 mM | 0.9602 mL | 4.8008 mL | 9.6015 mL |
| 10 mM | 0.4801 mL | 2.4004 mL | 4.8008 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet