β-Endorphin (human) (trifluoroacetate salt)
(Synonyms: β-Lipotropin (61-91)) 目录号 : GC48300
A neuropeptide with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
β-Endorphin is an endogenous 31-amino acid neuropeptide and opioid receptor agonist that binds to μ-, δ-, and κ-opioid receptors (Kis = 3.73, 5.02, and 32.7 nM, respectively, in COS-1 cells expressing rat receptors).1 It binds to rat and mouse brain membrane preparations (IC50s = 0.33 and 0.67 nM, respectively) and induces chemotaxis of human monocytes in vitro when used at a concentration of 0.1 pM.2,3,4 Intracerebroventricular administration of β-endorphin increases the latency to tail withdrawal in response to thermal stimuli in mice with a median antinociceptive dose (AD50) value of 27 pmol per animal.2 It also increases preference for the drug-paired place in a conditioned place preference test when administered to rats intracerebroventricularly at a dose of 5 μg.5
1.Mansour, A., Hoversten, M.T., Taylor, L.P., et al.The cloned μ, δ and κ receptors and their endogenous ligands: Evidence for two opioid peptide recognition coresBrain Res.700(1-2)89-98(1995) 2.Hammonds, R.G., Jr., Nicolas, P., and Li, C.H.β-Endorphin-(1-27) is an antagonist of β-endorphin analgesiaProc. Natl. Acad. Sci. U.S.A.81(5)1389-1390(1984) 3.GarzÓn, J., and SÁnchez-BlÁzquez, P.αN-acetyl derivatives of β-endorphin-(1-31) and -(1-27) regulate the supraspinal antinociceptive activity of different opioids in miceLife Sci.48(14)1417-1427(1991) 4.Sacerdote, P., and Panerai, A.E.Analysis of the beta-endorphin structure-related activity on human monocyte chemotaxis: Importance of the N- and C-terminalPeptides10(3)565-569(1989) 5.Bals-Kubik, R., Herz, A., and Shippenberg, T.S.β-endorphin-(1-27) is a naturally occurring antagonist of the reinforcing effects of opioidsNaunyn Schmiedebergs Arch. Pharmacol.338(4)392-396(1988)
Cas No. | N/A | SDF | |
别名 | β-Lipotropin (61-91) | ||
Canonical SMILES | [H]N[C@H](C(NCC(NCC(N[C@H](C(N[C@H](C(N[C@]([C@@H](C)O)([H])C(N[C@@H](CO)C(N[C@@H](CCC(O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CO)C(N[C@@H](CCC(N)=O)C(N[C@]([C@@H](C)O)([H])C(N1CCC[C@H]1C(N[C@@H](CC(C)C)C(N[C@@H](C(C)C)C(N[C@]([C@@H](C)O)([H])C(N[C@@H](CC(C)C)C(N[C@H](C(N[C@@H](CCCCN)C(N[C@@H](CC(N)=O)C(N[C@H](C(N[C@]([C@H](CC)C)([H])C(N[C@]([C@H](CC)C)([H])C(N[C@@H](CCCCN)C(N[C@@H](CC(N)=O)C(N[C@H](C(N[C@H](C(N[C@@H](CCCCN)C(N[C@@H](CCCCN)C(NCC(N[C@@H](CCC(O)=O)C(O)=O)=O)=O)=O)=O)CC2=CC=C(O)C=C2)=O)C)=O)=O)=O)=O)=O)C)=O)=O)=O)CC3=CC=CC=C3)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)CCSC)=O)CC4=CC=CC=C4)=O)=O)=O)CC5=CC=C(O)C=C5.FC(F)(C(O)=O)F | ||
分子式 | C158H251N39O46S.XCF3COOH | 分子量 | 3465 |
溶解度 | Water: 1 mg/ml | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 0.2886 mL | 1.443 mL | 2.886 mL |
5 mM | 0.0577 mL | 0.2886 mL | 0.5772 mL |
10 mM | 0.0289 mL | 0.1443 mL | 0.2886 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet