A-779 (trifluoroacetate salt)
目录号 : GC46766
A neuropeptide with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
A-779 is a peptide antagonist of the Mas receptor, also known as the angiotensin (1-7) (Ang (1-7)) receptor (IC50 = 0.3 nM in a radioligand binding assay).1 It does not compete with angiotensin 1 (AT1) and AT2 receptor agonists for binding in adrenocortical membranes when used at a concentration of 1 µM and exhibits an IC50 value greater than 10 µM in adrenomedullary membranes.2 In vitro, A-779 inhibits Ang (1-7)-induced release of arachidonic acid from CHO cells transfected with Mas.1 A-779 (0.01 mg/kg) prevents the antidiuretic effects of Ang (1-7) in water-loaded rats. It also inhibits the Ang (1-7)-induced decrease in mean arterial pressure (MAP) when administered by microinjection into the nucleus of the solitary tract with no effect on basal MAP. A-779 increases urine flow rate and sodium excretion in male, but not female, rats when AT1 and AT2 receptors are blocked by the selective antagonists losartan and PD 123319 , respectively.3
1.Santos, R.A., Simoes e Silva, A.C., Maric, C., et al.Angiotensin-(1-7) is an endogenous ligand for the G protein-coupled receptor MasProc. Natl. Acad. Sci. U.S.A.100(14)8258-8263(2003) 2.Santos, R.A., Campagnole-Santos, M.J., Baracho, N.C., et al.Characterization of a new angiotensin antagonist selective for angiotensin-(1-7): Evidence that the actions of angiotensin-(1-7) are mediated by specific angiotensin receptorsBrain Res. Bull.35(4)293-298(1994) 3.Mansoori, A.O., S., and Nematbakhsh, M.Role of Mas receptor antagonist (A779) on pressure diuresis and natriuresis and renal blood flow in the absence of angiotensin II receptors type 1 and 2 in female and male ratsJ. Physiol. Pharmacol.65(5)633-639(2014)
| Cas No. | N/A | SDF | |
| Canonical SMILES | O=C([C@H](CC1=CC=C(O)C=C1)NC([C@@H](NC([C@@H](NC([C@H](CC(O)=O)N)=O)CCCNC(N)=N)=O)C(C)C)=O)N[C@]([H])(C(N[C@@H](CC2=CNC=N2)C(N[C@@H](C(O)=O)C)=O)=O)[C@H](CC)C.FC(F)(C(O)=O)F | ||
| 分子式 | C39H60N12O11.XCF3COOH | 分子量 | 873 |
| 溶解度 | DMF: 10 mg/ml,DMSO: 33 mg/ml,PBS (pH 7.2): 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1455 mL | 5.7274 mL | 11.4548 mL |
| 5 mM | 0.2291 mL | 1.1455 mL | 2.291 mL |
| 10 mM | 0.1145 mL | 0.5727 mL | 1.1455 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。